Immunogenetics

, Volume 47, Issue 1, pp 77–87

Structural analysis and chromosomal localization of the mouse Psmb5 gene coding for the constitutively expressed β-type proteasome subunit

Authors

  • Keiko Kohda
    • Department of Biochemistry, Hokkaido University School of Medicine, Sapporo 060, Japan
  • Yoichi Matsuda
    • Laboratory of Animal Genetics, Nagoya University School of Agricultural Sciences, Nagoya 464–01, Japan
  • Teruo Ishibashi
    • Department of Biochemistry, Hokkaido University School of Medicine, Sapporo 060, Japan
  • Keiji Tanaka
    • Tokyo Metropolitan Institute of Medical Science, Tokyo 113, Japan
  • M. Kasahara
    • Department of Biochemistry, Hokkaido University School of Medicine, Sapporo 060, Japan
ORIGINAL PAPER

DOI: 10.1007/s002510050329

Cite this article as:
Kohda, K., Matsuda, Y., Ishibashi, T. et al. Immunogenetics (1997) 47: 77. doi:10.1007/s002510050329

Abstract

 The proteasome is a multi-subunit protease responsible for the production of peptides presented by major histocompatibility complex class I molecules. Accumulated evidence indicates that, upon stimulation with interferon-γ (IFN-γ), three β-type subunits, designated LMP2, LMP7, and PSMB10, are incorporated into the 20S proteasome by displacing the housekeeping β-type subunits designated PSMB6, PSMB5, and PSMB7, respectively. These changes in the subunit composition appear to facilitate class I-mediated antigen presentation, presumably by altering the cleavage specificities of the proteasome. In the present study, we determined the organization of the mouse gene Psmb5, coding for the PSMB5 subunit. Psmb5 is made up of three exons, spanning ∼5 kilobases. Its exon-intron organization differs radically from those of the other IFN-γ-regulated, β-type subunit genes including Lmp7 with which Psmb5 is believed to share an immediate common ancestor. The structure of the mouse Psmb5 gene is identical to that of its recently characterized human counterpart. Thus, the unique organization of the gene coding for the PSMB5 subunit appears to have been established before mammalian radiation. As well as the Psmb5 gene, the mouse genome contains a processed pseudogene designated Psmb5-ps. Interspecific backcross mapping showed that Psmb5 maps close to the Gtrgal2 locus on chromosome 14 and that Psmb5-ps is located in the vicinity of the Psme3 locus on chromosome 11. These results were confirmed by fluorescent in situ hybridization analysis that localized Psmb5 to band C2 to proximal D1 of chromosome 14 and Psmb5-ps to band D of chromosome 11.

Key words ProteasomePsmb5X subunitGene structureProteasome activator

Copyright information

© Springer-Verlag Berlin Heidelberg 1997