ORIGINAL PAPER

Immunogenetics

, Volume 46, Issue 3, pp 199-205

First online:

Sequence-based association analysis of HLA class I and II alleles in Japanese supports conservation of common haplotypes

  • K. TokunagaAffiliated withDepartment of Human Genetics, Graduate School of International Health, University of Tokyo, Tokyo, Japan
  • , Yoshihide IshikawaAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
  • , Atsuko OgawaAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
  • , Huiru WangAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
  • , Shigeki MitsunagaAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
  • , Satoshi MoriyamaAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
  • , Ling LinAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
  • , Makoto BannaiAffiliated withTokyo Metropolitan Blood Center, Tokyo, Japan
  • , Yoshihisa WatanabeAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
    • , Kouichi KashiwaseAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
    • , Hidenori TanakaAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
    • , Tatsuya AkazaAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
    • , Kenji TadokoroAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan
    • , Takeo JujiAffiliated withJapanese Red Cross Central Blood Center, Tokyo, Japan

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Abstract

 Alleles of HLA-A, B, C, DRB1, DQB1, and DPB1 loci were fully determined in 117 healthy Japanese. A * 2402, A * 3303, A * 1101, A * 0201, B * 4403, B * 5201, Cw * 0102, Cw * 1403, Cw * 0304, Cw * 0702, Cw * 0801, and Cw * 1202 showed frequencies of over 10%. Multi-locus haplotype frequencies were estimated by the maximum likelihood method. Strength of association between C and B loci was comparable with that between DRB1 and DQB1 loci. Alleles unidentified by a serological method and having very similar nucleotide sequences (A2: A * 0201, A * 0206, A * 0207, B61: B * 4002, B * 4006) were carried by different haplotypes. Several frequent five-locus haplotypes were identified including A * 3303-Cw * 1403-B * 4403-DRB1 * 1302-DQB1 * 0604, and A * 2402-Cw * 1202-B * 5201-DRB1 * 1502-DQB1 * 0601. These sequence-based haplotypes corresponded to serology-based common haplotypes which have already been described in Japanese. These findings indicate that common HLA haplotypes consist of particular sets of HLA alleles and that these haplotypes have been conserved through recent human evolution.