Immunogenetics

, Volume 46, Issue 3, pp 199–205

Sequence-based association analysis of HLA class I and II alleles in Japanese supports conservation of common haplotypes

Authors

  • K. Tokunaga
    • Department of Human Genetics, Graduate School of International Health, University of Tokyo, Tokyo, Japan
  • Yoshihide Ishikawa
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Atsuko Ogawa
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Huiru Wang
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Shigeki Mitsunaga
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Satoshi Moriyama
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Ling Lin
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Makoto Bannai
    • Tokyo Metropolitan Blood Center, Tokyo, Japan
  • Yoshihisa Watanabe
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Kouichi Kashiwase
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Hidenori Tanaka
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Tatsuya Akaza
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Kenji Tadokoro
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
  • Takeo Juji
    • Japanese Red Cross Central Blood Center, Tokyo, Japan
ORIGINAL PAPER

DOI: 10.1007/s002510050262

Cite this article as:
Tokunaga, K., Ishikawa, Y., Ogawa, A. et al. Immunogenetics (1997) 46: 199. doi:10.1007/s002510050262

Abstract

 Alleles of HLA-A, B, C, DRB1, DQB1, and DPB1 loci were fully determined in 117 healthy Japanese. A * 2402, A * 3303, A * 1101, A * 0201, B * 4403, B * 5201, Cw * 0102, Cw * 1403, Cw * 0304, Cw * 0702, Cw * 0801, and Cw * 1202 showed frequencies of over 10%. Multi-locus haplotype frequencies were estimated by the maximum likelihood method. Strength of association between C and B loci was comparable with that between DRB1 and DQB1 loci. Alleles unidentified by a serological method and having very similar nucleotide sequences (A2: A * 0201, A * 0206, A * 0207, B61: B * 4002, B * 4006) were carried by different haplotypes. Several frequent five-locus haplotypes were identified including A * 3303-Cw * 1403-B * 4403-DRB1 * 1302-DQB1 * 0604, and A * 2402-Cw * 1202-B * 5201-DRB1 * 1502-DQB1 * 0601. These sequence-based haplotypes corresponded to serology-based common haplotypes which have already been described in Japanese. These findings indicate that common HLA haplotypes consist of particular sets of HLA alleles and that these haplotypes have been conserved through recent human evolution.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997