Immunogenetics

, Volume 51, Issue 8, pp 659–669

Genomic organization of the human leukocyte immunoglobulin-like receptors within the leukocyte receptor complex on Chromosome 19q13.4

  • W. Robert Liu
  • Joomyeong Kim
  • Chioma Nwankwo
  • Linda K. Ashworth
  • J. P. Arm
Original paper

DOI: 10.1007/s002510000183

Cite this article as:
Liu, W., Kim, J., Nwankwo, C. et al. Immunogenetics (2000) 51: 659. doi:10.1007/s002510000183

Abstract

 The leukocyte immunoglobulin (Ig)-like receptors (LIRs) comprise a family of cell surface receptors that couple to either activating or inhibitory signals depending on the nature of their transmembrane and cytoplasmic domains. We describe the organization and fine localization of the genes for LIR-1 and LIR-5, which are inhibitory receptors, and LIR-6, which is an activating receptor. The genomic organization of all three genes is highly conserved from the signal peptide through the membrane-proximal Ig domain but diverges thereafter depending on the inhibitory or activating nature of the gene product. The 3′ untranslated region of the gene for LIR-6 contains a 37-base pair repeat not present in the LIR-1 or LIR-5 genes. 5′ rapid amplification of cDNA ends defined the putative transcription initiation site of the LIR-5 gene, which is TATA-less. A nucleotide substitution in the LIR-5 gene led to loss of an intron present in the 5′ untranslated region of the LIR-1 and LIR-6 genes. Differences in the genomic structure of these three LIR genes suggests possible mechanisms for their differential expression in cells of hematopoietic lineage. The three genes are in a region of Chromosome 19q13.4 that is immediately centromeric of the killer cell Ig-like receptor genes and are separated from one another by ∼20 to 30 kb, suggesting that they arose by gene duplication from a common ancestor.

Key words Leukocyte Ig-like receptors ILT Human Chromosome 19 Immunoglobulin superfamily Gene structure 

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • W. Robert Liu
    • 1
  • Joomyeong Kim
    • 3
  • Chioma Nwankwo
    • 1
  • Linda K. Ashworth
    • 3
  • J. P. Arm
    • 1
  1. 1.Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, One Jimmy Fund Way, Boston, MA 02115, USA e-mail: jarm@rics.bwh.harvard.edu Tel.: +1-617-5251305 Fax: +1-617-5251310US
  2. 2.The Partners Asthma Center, Brigham and Women's Hospital, Boston, MA 02115, USAUS
  3. 3.The Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94550, USAUS