Immunogenetics

, Volume 65, Issue 5, pp 345–355

Allelic variants in TLR10 gene may influence bilateral affectation and clinical course of Meniere’s disease

  • Teresa Requena
  • Irene Gazquez
  • Antonia Moreno
  • Angel Batuecas
  • Ismael Aran
  • Andres Soto-Varela
  • Sofia Santos-Perez
  • Nicolas Perez
  • Herminio Perez-Garrigues
  • Alicia Lopez-Nevot
  • Eduardo Martin
  • Ricardo Sanz
  • Paz Perez
  • Gabriel Trinidad
  • Marta E. Alarcon-Riquelme
  • Roberto Teggi
  • Laura Zagato
  • Miguel A. Lopez-Nevot
  • Jose A. Lopez-Escamez
Original Paper

DOI: 10.1007/s00251-013-0683-z

Cite this article as:
Requena, T., Gazquez, I., Moreno, A. et al. Immunogenetics (2013) 65: 345. doi:10.1007/s00251-013-0683-z

Abstract

Toll-like receptors trigger the innate immune response by activating various cell types such us macrophages and lymphocytes. We genotyped SNV of TLR3, TRL7, TLR8 and TLR10 in 863 Spanish and 150 Italian patients with Meniere’s disease (MD) and 1,013 controls by using Taqman assays. Real-Time qPCR was used to measure the expression level of TLR10 in peripheral blood leukocytes. The overall dataset showed that the C allele and the CC genotype of rs11096955 in TLR10 gene were more commonly observed in controls than patients (corrected p = 1 × 10−3, OR = 0.68 [95 % confidence interval, 0.54–0.84] for CC genotype; corrected p = 1.5 × 10−5, OR = 0.75 [0.66–0.85] for allele C). Moreover, the CC genotype was more frequent in patients with uni- (19 %) than bilateral sensorineural hearing loss (SNHL) (13 %). Logistic regression demonstrated that the time since the onset of MD, Tumarkin crises, hearing stage and rs11096955 were independent factors influencing the risk of bilateral SNHL. In addition, rs11096955 influenced hearing loss progression in patients with bilateral MD. No change in expression of TLR10 was observed according to CC, CA or AA genotypes. Our data suggest that allelic variants of TLR10 gene may influence the susceptibility and time-course of hearing loss of MD in the European population.

Keywords

Mènière’s disease Toll-like receptor Single-nucleotide polymorphisms Sensorineural hearing loss 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Teresa Requena
    • 1
  • Irene Gazquez
    • 1
    • 2
  • Antonia Moreno
    • 2
  • Angel Batuecas
    • 3
  • Ismael Aran
    • 4
  • Andres Soto-Varela
    • 5
  • Sofia Santos-Perez
    • 5
  • Nicolas Perez
    • 6
  • Herminio Perez-Garrigues
    • 7
  • Alicia Lopez-Nevot
    • 8
  • Eduardo Martin
    • 9
  • Ricardo Sanz
    • 9
  • Paz Perez
    • 10
  • Gabriel Trinidad
    • 11
  • Marta E. Alarcon-Riquelme
    • 12
  • Roberto Teggi
    • 13
  • Laura Zagato
    • 14
  • Miguel A. Lopez-Nevot
    • 2
  • Jose A. Lopez-Escamez
    • 1
    • 15
  1. 1.Otology and Neurotology Group CTS495, Human DNA Variability Department, Centro de Genómica e Investigación OncológicaPfizer/Universidad de Granada/Junta de Andalucía (GENYO)GranadaSpain
  2. 2.Department of ImmunologyHospital Virgen de las NievesGranadaSpain
  3. 3.Department of OtolaryngologyHospital Universitario de SalamancaSalamancaSpain
  4. 4.Department of OtolaryngologyComplexo Hospitalario de PontevedraPontevedraSpain
  5. 5.Division of Otoneurology, Department of OtorhinolaryngologyComplexo Hospitalario UniversitarioSantiago de CompostelaSpain
  6. 6.Department of OtolaryngologyClinica Universidad de NavarraPamplonaSpain
  7. 7.Division Otoneurology, Department of OtorhinolaryngologyHospital La FeValenciaSpain
  8. 8.Department of OtolaryngologyHospital Virgen de las NievesGranadaSpain
  9. 9.Department OtolaryngologyHospital Universitario de GetafeMadridSpain
  10. 10.Department of OtolaryngologyHospital de CabueñesGijónSpain
  11. 11.Department of OtorhinolaryngologyComplejo Hospitalario BadajozBadajozSpain
  12. 12.Group of Genetics of Complex Diseases, Human DNA Variability Department, Centro de Genómica e Investigación OncológicaPfizer/Universidad de Granada/Junta de Andalucía (GENYO)GranadaSpain
  13. 13.Department of OtolaryngologySan Raffaelle Scientific InstituteMilanItaly
  14. 14.Division of Dialysis and NephrologyHospital San Raffaelle Scientic InstituteMilanItaly
  15. 15.Department of OtolaryngologyHospital de PonienteAlmeriaSpain