Immunogenetics

, Volume 65, Issue 1, pp 37–46

Characterisation of four major histocompatibility complex class II genes of the koala (Phascolarctos cinereus)

  • Quintin Lau
  • Sarah E. Jobbins
  • Katherine Belov
  • Damien P. Higgins
Original Paper

DOI: 10.1007/s00251-012-0658-5

Cite this article as:
Lau, Q., Jobbins, S.E., Belov, K. et al. Immunogenetics (2013) 65: 37. doi:10.1007/s00251-012-0658-5

Abstract

Major histocompatibility complex (MHC) class II molecules have an integral role in the adaptive immune response, as they bind and present antigenic peptides to T helper lymphocytes. In this study of koalas, species-specific primers were designed to amplify exon 2 of the MHC class II DA and DB genes, which contain much of the peptide-binding regions of the α and β chains. A total of two DA α1 domain variants and eight DA β1 (DAB), three DB α1 and five DB β1 variants were amplified from 20 koalas from two free-living populations from South East Queensland and the Port Macquarie region in northern New South Wales. We detected greater variation in the β1 than in the α1 domains as well as evidence of positive selection in DAB. The present study provides a springboard to future investigation of the role of MHC in disease susceptibility in koalas.

Keywords

KoalaPhascolarctos cinereusMHC class IIMarsupialDiversity

Supplementary material

251_2012_658_MOESM1_ESM.pdf (792 kb)
ESM 1(PDF 792kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Quintin Lau
    • 1
  • Sarah E. Jobbins
    • 1
  • Katherine Belov
    • 1
  • Damien P. Higgins
    • 1
  1. 1.Faculty of Veterinary ScienceThe University of SydneyNSWAustralia