Immunogenetics

, Volume 65, Issue 1, pp 63–73

The chicken SLAM family

Authors

  • Christian Straub
    • Institute for Animal Physiology, Department of Veterinary SciencesUniversity of Munich
  • Birgit C. Viertlboeck
    • Institute for Animal Physiology, Department of Veterinary SciencesUniversity of Munich
    • Institute for Animal Physiology, Department of Veterinary SciencesUniversity of Munich
Original Paper

DOI: 10.1007/s00251-012-0657-6

Cite this article as:
Straub, C., Viertlboeck, B.C. & Göbel, T.W. Immunogenetics (2013) 65: 63. doi:10.1007/s00251-012-0657-6

Abstract

The signaling lymphocytic activation molecule (SLAM) family of receptors is critically involved in the immune regulation of lymphocytes but has only been detected in mammals, with one member being present in Xenopus. Here, we describe the identification, cloning, and analysis of the chicken homologues to the mammalian SLAMF1 (CD150), SLAMF2 (CD48), and SLAMF4 (CD244, 2B4). Two additional chicken SLAM genes were identified and designated SLAMF3like and SLAM5like in order to stress that those two receptors have no clear mammalian counterpart but share some features with mammalian SLAMF3 and SLAMF5, respectively. Three of the chicken SLAM genes are located on chromosome 25, whereas two are currently not yet assigned. The mammalian and chicken receptors share a common structure with a V-like domain that lacks conserved cysteine residues and a C2-type Ig domain with four cysteines forming two disulfide bonds. Chicken SLAMF2, like its mammalian counterpart, lacks a transmembrane and cytoplasmic domain and thus represents a glycosyl-phosphatidyl-inositol-anchored protein. The cytoplasmic tails of SLAMF1 and SLAMF4 display two and four conserved immunoreceptor tyrosine-based switch motifs (ITSMs), respectively, whereas both chicken SLAMF3like and SLAMF5like have only a single ITSM. We have also identified the chicken homologues of the SLAM-associated protein family of adaptors (SAP), SAP and EAT-2. Chicken SAP shares about 70 % identity with mammalian SAP, and chicken EAT-2 is homologous to mouse EAT-2, whereas human EAT-2 is much shorter. The characterization of the chicken SLAM family of receptors and the SAP adaptors demonstrates the phylogenetic conservation of this family, in particular, its signaling capacities.

Keywords

SLAM family Chicken EAT-2 SAP

Abbreviations

GPI

glycosyl-phosphatidyl-inositol

SLAM

signaling lymphocytic activation molecule

SAP

SLAM-associated protein family of adaptors

ITSM

immunoreceptor tyrosine-based switch motif

Supplementary material

251_2012_657_MOESM1_ESM.tif (1.1 mb)
High resolution image file (TIFF 1108 kb)
251_2012_657_MOESM2_ESM.doc (56 kb)
ESM 2 (DOC 56 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012