Immunogenetics

, Volume 64, Issue 1, pp 59–69

Systemic response to Campylobacter jejuni infection by profiling gene transcription in the spleens of two genetic lines of chickens

  • Xianyao Li
  • Christina L. Swaggerty
  • Michael H. Kogut
  • Hsin-I Chiang
  • Ying Wang
  • Kenneth J. Genovese
  • Haiqi He
  • Fiona M. McCarthy
  • Shane C. Burgess
  • Igal Y. Pevzner
  • Huaijun Zhou
Original Paper

DOI: 10.1007/s00251-011-0557-1

Cite this article as:
Li, X., Swaggerty, C.L., Kogut, M.H. et al. Immunogenetics (2012) 64: 59. doi:10.1007/s00251-011-0557-1

Abstract

Campylobacter jejuni (C. jejuni) is a leading cause of human bacterial enteritis worldwide with poultry products being a major source of C. jejuni contamination. The chicken is the natural reservoir of C. jejuni where bacteria colonize the digestive tract of poultry, but rarely cause symptoms of disease. To understand the systemic molecular response mechanisms to C. jejuni infection in chickens, total splenic RNA was isolated and applied to a whole genome chicken microarray for comparison between infected (I) and non-infected (N) chickens within and between genetic lines A and B. There were more total splenic host genes responding to the infection in resistant line A than in susceptible line B. Specifically, genes for lymphocyte activation, differentiation and humoral response, and Ig light and heavy chain were upregulated in the resistant line. In the susceptible line, genes for regulation of erythrocyte differentiation, hemopoiesis, and RNA biosynthetic process were all downregulated. An interaction analysis between genetic lines and treatment demonstrated distinct defense mechanisms between lines: the resistant line promoted apoptosis and cytochrome c release from mitochondria, whereas the susceptible line responded with a downregulation of both functions. This was the first time that such systemic defensive mechanisms against C. jejuni infection have been reported. The results of this study revealed novel molecular mechanisms of the systemic host responses to C. jejuni infection in chickens that warrant further investigation.

Keywords

Chicken C. jejuni Transcriptome Resistant Susceptible 

Supplementary material

251_2011_557_MOESM1_ESM.xls (54 kb)
Supplemental file 1Overlapped genes between AI/AN and BI/BN. This table provides a list of overlapped genes between AI/AN and BI/BN (XLS 54 kb)
251_2011_557_MOESM2_ESM.xls (38 kb)
Supplemental file 2Fold enrichment for a significant gene in the comparison of AI/AN. This table shows the enriched GO BP terms for the differentially expressed genes in the comparison of AI/AN (XLS 38 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Xianyao Li
    • 1
    • 5
  • Christina L. Swaggerty
    • 2
  • Michael H. Kogut
    • 2
  • Hsin-I Chiang
    • 1
  • Ying Wang
    • 1
  • Kenneth J. Genovese
    • 2
  • Haiqi He
    • 2
  • Fiona M. McCarthy
    • 3
  • Shane C. Burgess
    • 3
  • Igal Y. Pevzner
    • 4
  • Huaijun Zhou
    • 1
  1. 1.Department of Poultry ScienceTexas A&M UniversityCollege StationUSA
  2. 2.United States Department of Agriculture, Agricultural Research ServiceSouthern Plains Agricultural Research CenterCollege StationUSA
  3. 3.Department of Basic Sciences, College of Veterinary MedicineMississippi State UniversityStarkvilleUSA
  4. 4.Cobb-Vantress, Inc.Siloam SpringsUSA
  5. 5.College of Animal Science and TechnologyShandong Agricultural UniversityTaianChina

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