Immunogenetics

, Volume 61, Issue 8, pp 565–579

Identification of natural killer cell receptor clusters in the platypus genome reveals an expansion of C-type lectin genes

  • Emily S. W. Wong
  • Claire E. Sanderson
  • Janine E. Deakin
  • Camilla M. Whittington
  • Anthony T. Papenfuss
  • Katherine Belov
Original Paper

DOI: 10.1007/s00251-009-0386-7

Cite this article as:
Wong, E.S.W., Sanderson, C.E., Deakin, J.E. et al. Immunogenetics (2009) 61: 565. doi:10.1007/s00251-009-0386-7

Abstract

Natural killer (NK) cell receptors belong to two unrelated, but functionally analogous gene families: the immunoglobulin superfamily, situated in the leukocyte receptor complex (LRC) and the C-type lectin superfamily, located in the natural killer complex (NKC). Here, we describe the largest NK receptor gene expansion seen to date. We identified 213 putative C-type lectin NK receptor homologs in the genome of the platypus. Many have arisen as the result of a lineage-specific expansion. Orthologs of OLR1, CD69, KLRE, CLEC12B, and CLEC16p genes were also identified. The NKC is split into at least two regions of the genome: 34 genes map to chromosome 7, two map to a small autosome, and the remainder are unanchored in the current genome assembly. No NK receptor genes from the LRC were identified. The massive C-type lectin expansion and lack of Ig-domain-containing NK receptors represents the most extreme polarization of NK receptors found to date. We have used this new data from platypus to trace the possible evolutionary history of the NK receptor clusters.

Keywords

NKCPlatypusNatural killer receptorsEvolutionGene expansionImmune

Supplementary material

251_2009_386_MOESM1_ESM.doc (755 kb)
ESM 1Includes detailed information on the identification of Ig domains, specifically pertaining to the identification of OSCAR, SIGLEC and Fc receptor genes, FISH data and accession numbers for all non-platypus sequences used in phylogenetic analyses (DOC 755 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Emily S. W. Wong
    • 1
  • Claire E. Sanderson
    • 1
  • Janine E. Deakin
    • 2
  • Camilla M. Whittington
    • 1
  • Anthony T. Papenfuss
    • 3
  • Katherine Belov
    • 1
  1. 1.Faculty of Veterinary ScienceUniversity of SydneySydneyAustralia
  2. 2.Research School of Biological SciencesAustralian National UniversityCanberraAustralia
  3. 3.Bioinformatics DivisionThe Walter and Eliza Hall Institute for Medical ResearchParkvilleAustralia