Immunogenetics

, Volume 61, Issue 8, pp 581–596

On the genomics of immunoglobulins in the gray, short-tailed opossum Monodelphis domestica

Original Paper

DOI: 10.1007/s00251-009-0385-8

Cite this article as:
Wang, X., Olp, J.J. & Miller, R.D. Immunogenetics (2009) 61: 581. doi:10.1007/s00251-009-0385-8

Abstract

Annotated maps of the IGH, IGK, and IGL loci in the gray, short-tailed opossum Monodelphis domestica were generated from analyses of the available whole genome sequence for this species. Analyses of their content and organization confirmed a number of previous conclusions based on characterization of complementary DNAs encoding opossum immunoglobulin heavy and light chains and limited genomic analysis, including (a) the predominance of a single immunoglobulin heavy chain variable region (IGHV) subgroup and clan, (b) the presence of a single immunoglobulin (Ig)G subclass, (c) the apparent absence of an IgD, and (d) the general organization and V gene complexity of the IGK and IGL light chain loci. In addition, several unexpected discoveries were made including the presence of a partial V to D, germline-joined IGHV segment, the first germline-joined Ig V gene to be found in a mammal. In addition was the presence of a larger number of IGKV subgroups than had been previously identified. With this report, annotated maps of the major histocompatibility complex, T-cell receptor, and immunoglobulin loci have been completed for M. domestica, the only non-eutherian mammalian species for which this has been accomplished, strengthening the utility of this species as a model organism.

Keywords

Immunoglobulins Genomics Marsupials Monodelphis domestica 

Supplementary material

251_2009_385_MOESM1_ESM.doc (204 kb)
Supplementary Table 1Locations of IGH gene segments in the MonDom5 assembly (DOC 204 kb)
251_2009_385_MOESM2_ESM.doc (264 kb)
Supplementary Table 2Locations of IGK gene segments in the MonDom5 assembly (DOC 264 kb)
251_2009_385_MOESM3_ESM.doc (202 kb)
Supplementary Table 3Locations of IGL gene segments in the MonDom5 assembly (DOC 202 kb)
251_2009_385_MOESM4_ESM.ppt (53 kb)
Supplementary Figure 1(PPT 53 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Xinxin Wang
    • 1
  • Jonathan J. Olp
    • 1
  • Robert D. Miller
    • 1
  1. 1.Department of Biology, Center for Evolutionary & Theoretical ImmunologyThe University of New MexicoAlbuquerqueUSA

Personalised recommendations