Immunogenetics

, 60:699

Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis

Original Paper

DOI: 10.1007/s00251-008-0326-y

Cite this article as:
Qi, Z.T. & Nie, P. Immunogenetics (2008) 60: 699. doi:10.1007/s00251-008-0326-y

Abstract

Using bioinformatics approach, the genome locus containing interleukin (IL)-22, IL-26, and interferon gamma (IFN-γ) genes has been identified in the amphibian, Xenopus tropicalis. Like that in other vertebrates such as fish, birds, and mammals, the Xenopus IL-22, IL-26, and IFN-γ are clustered in the same chromosome and the adjacent genes are conserved. The genomic structures of the Xenopus IL-22, IL-26, and IFN-γ gene were identical to that of their mammalian counterparts. The Xenopus IL-22 and IL-26 genes contained five exons and four introns while the Xenopus IFN-γ gene consisted of four exons and three introns. The Xenopus IL-22, IL-26, and IFN-γ share 14.1–41.6%, 14.6–31.2%, and 23.7–36.5% identity to their counterparts in other species, respectively. Reverse-transcription polymerase chain reaction (PCR) and real-time quantitative PCR analyses revealed that the expression of IL-22, IL-26, and IFN-γ genes was significantly upregulated after simulation with bacterial polyliposaccharide and/or synthetic double-stranded poly(I:C), suggesting these cytokines like those in other vertebrates play an important role in regulating immune response in Xenopus.

Keywords

Interleukin 22 Interleukin 26 Interferon gamma Xenopus tropicalis 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.State Key Laboratory of Freshwater Ecology and BiotechnologyChinese Academy of SciencesWuhanChina
  2. 2.Laboratory of Fish Diseases, Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  3. 3.Graduate School of Chinese Academy of SciencesBeijingChina

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