Immunogenetics

, Volume 57, Issue 11, pp 828–836

Immunologic significance of HLA class I genes in measles virus-specific IFN-γ and IL-4 cytokine immune responses

  • Inna G. Ovsyannikova
  • Jenna E. Ryan
  • Robert A. Vierkant
  • V. Shane Pankratz
  • Robert M. Jacobson
  • Gregory A. Poland
Original Paper

DOI: 10.1007/s00251-005-0061-6

Cite this article as:
Ovsyannikova, I.G., Ryan, J.E., Vierkant, R.A. et al. Immunogenetics (2005) 57: 828. doi:10.1007/s00251-005-0061-6

Abstract

The variability of immune responses modulated by human leukocyte antigen (HLA) genes and secreted cytokines is a significant factor in the development of a protective effect of measles vaccine. We studied the association between type 1 helper T cells (Th1)- and Th2-like cytokine immune responses and HLA class I alleles among 339 schoolchildren who previously received two doses of the measles vaccine. Median values for measles-specific interferon gamma (IFN-γ) and interleukin-4 (IL-4) cytokines were 40.7 pg/ml [interquartile range (IQR) 8.1–176.7] and 9.7 pg/ml (IQR 2.8–24.3), respectively. Class I HLA-A (*0101 and *3101) and HLA-Cw (*0303 and *0501) alleles were significantly associated with measles-virus-induced IFN-γ secretion. HLA-A*3101 and Cw*0303 were associated with a higher median IFN-γ response, while A*0101 and Cw*0501 were associated with lower measles-specific IFN-γ response. We found limited associations between HLA class I gene polymorphisms and Th2-like (IL-4) immune responses after measles vaccination, indicating that HLA class I molecules may have a limited effect on measles-vaccine-induced IL-4 secretion. Understanding the genetic factors that influence variations in cytokine secretion following measles vaccination will provide insight into the factors that influence both cell-mediated and humoral immunity to measles.

Keywords

Measles virus Measles vaccine HLA class I genes IFN-γ and IL-4 cytokine responses PBMC T cells 

Abbreviations

CTL

Cytotoxic T lymphocyte

HLA

human leukocyte antigen

PBMC

peripheral blood mononuclear cells

PHA

phytohemagglutinin

RSCA

reference strand conformation analysis

SSP

sequence-specific primer

IFN-γ

interferon gamma

IL-4

interleukin-4

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Inna G. Ovsyannikova
    • 1
  • Jenna E. Ryan
    • 1
  • Robert A. Vierkant
    • 2
  • V. Shane Pankratz
    • 2
  • Robert M. Jacobson
    • 1
    • 3
  • Gregory A. Poland
    • 1
    • 4
  1. 1.Mayo Vaccine Research GroupMayo Clinic College of MedicineRochesterUSA
  2. 2.Department of Health Science ResearchMayo Clinic College of MedicineRochesterUSA
  3. 3.Department of Pediatric and Adolescent MedicineMayo Clinic College of MedicineRochesterUSA
  4. 4.Program in Translational Immunovirology and BiodefenseMayo Clinic College of MedicineRochesterUSA

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