Immunogenetics

, Volume 56, Issue 11, pp 833–839

Molecular cloning of KLRI1 and KLRI2, a novel pair of lectin-like natural killer-cell receptors with opposing signalling motifs

  • Per C. Saether
  • Ingunn H. Westgaard
  • Line M. Flornes
  • Sigurd E. Hoelsbrekken
  • James C. Ryan
  • Sigbjørn Fossum
  • Erik Dissen
Original Paper

DOI: 10.1007/s00251-004-0759-x

Cite this article as:
Saether, P.C., Westgaard, I.H., Flornes, L.M. et al. Immunogenetics (2005) 56: 833. doi:10.1007/s00251-004-0759-x

Abstract

We here report the molecular cloning of a novel family of killer-cell lectin-like (KLR) receptors in the rat and the mouse, termed KLRI. In both species, there are two members, KLRI1 and KLRI2. While the extracellular lectin-like domains of KLRI1 and KLRI2 are similar [74% (rat) and 83% (mouse) amino acid identity], they differ intracellularly. KLRI1 has two immunoreceptor tyrosine-based inhibition motifs (ITIMs) in the cytoplasmic domain, suggesting an inhibitory function. KLRI2 has no ITIM, but a positively charged lysine residue in the transmembrane region, suggesting association with activating adapter molecules. Klri1 and Klri2 are localized within the natural killer (NK) cell gene complex on rat chromosome 4 and mouse chromosome 6. By RT-PCR and Northern blot analysis KLRI1 and KLRI2 were selectively expressed by NK cells in both rat and mouse. Epitope-tagged expression constructs of rat KLRI1 and rat KLRI2 induced surface expression of a nondisulphide-linked protein of Mr 36,000/39,000 and Mr 34,000, respectively.

Keywords

Natural killer cellsReceptorsSignalling motifs

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Per C. Saether
    • 1
  • Ingunn H. Westgaard
    • 1
  • Line M. Flornes
    • 1
  • Sigurd E. Hoelsbrekken
    • 1
  • James C. Ryan
    • 2
  • Sigbjørn Fossum
    • 1
  • Erik Dissen
    • 1
  1. 1.Department of Anatomy, Institute of Basic Medical SciencesUniversity of OsloOsloNorway
  2. 2.Veterans Administration Medical Center, Northern California Institute for Research and EducationUniversity of CaliforniaSan FranciscoUSA