Immunogenetics

, Volume 56, Issue 11, pp 781–787

HLA polymorphisms are associated with Helicobacter pylori infected gastric cancer in a high risk population, China

  • Zhaohui Li
  • Dafang Chen
  • Chunfeng Zhang
  • Yong Li
  • Bangwei Cao
  • Tao Ning
  • Yiming Zhao
  • Weicheng You
  • Yang Ke
Original Paper

DOI: 10.1007/s00251-004-0723-9

Cite this article as:
Li, Z., Chen, D., Zhang, C. et al. Immunogenetics (2005) 56: 781. doi:10.1007/s00251-004-0723-9

Abstract

Helicobacter pylori is one of the most common bacterial infections associated with an increased risk of gastric cancer, but its association with host factors, particularly polymorphisms of the immune response genes, such as human leukocyte antigen (HLA) genes, is still unclear. To investigate the role of HLA polymorphisms in the risk of gastric cancer among subjects with H. pylori infection, a case-control study involving 52 gastric cancer patients and 139 non-cancer controls was conducted in Linqu County, China, an area with a high incidence of gastric cancer. Polymorphisms of HLA class I and class II alleles were determined by PCR with sequence-specific primers (PCR-SSP). The information about H. pylori infection was obtained from previous records. Among 48 class I and 19 class II HLA alleles detected in this study, two alleles, CW*03 and DRB1*01, were found to be distributed significantly differently between patients and controls [odds ratio(OR)=1.95, 95% confidence interval (CI)=1.13–3.35, P=0.017 and OR=4.39, 95% CI=1.39–13.84, P=0.012, respectively). The OR of gastric cancer risk in individuals carrying CW*03/CW*03 or CW*03/CW*N was 2.06, 95% CI=1.05–4.02, P=0.035, while the OR was 3.49, 95% CI=1.0–12.4, P=0.04 for DRB1*01/DRB1*01 or DRB1*01/DRB1*N carriers. The analysis of the interaction between H. pylori infection and HLA risk genotypes of CW*03 or DRB1*01 revealed that the effect of CW*03 and DRB1*01 genotypes on gastric cancer risk was manifested stronger in H. pylori-positive individuals (OR=5.30, 95% CI=1.73–16.29, P=0.004 and OR=13.38, 95% CI=2.52–70.98, P=0.002, respectively) than in H. pylori-negative ones (OR=1.25, 95% CI=0.25–6.12, P=0.785 and OR=2.26, 95% CI=0.18–28.88, P=0.531, respectively). The combined effect of the two risk HLA genotypes on gastric cancer risk was also analysed. The result showed that the individuals carrying both the CW*03 and DRB1*01 alleles could only be found in cancer patients (5/52), and not in controls (0/139), further suggesting that CW*03 and DRB1*01 are risk alleles advancing the progression of tumorigenesis. These observations demonstrate that host HLA genotypes may play an important role in the risk of gastric cancer, especially among persons with H. pylori infection.

Keywords

Human leukocyte antigenPolymorphismHelicobacter pyloriGenetic susceptibilityGastric cancer

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Zhaohui Li
    • 1
  • Dafang Chen
    • 2
  • Chunfeng Zhang
    • 3
  • Yong Li
    • 3
  • Bangwei Cao
    • 3
  • Tao Ning
    • 3
  • Yiming Zhao
    • 4
  • Weicheng You
    • 3
  • Yang Ke
    • 3
    • 5
  1. 1.Department of General SurgeryQilu Hospital of Shandong UniversityJinanPeople’s Republic of China
  2. 2.Department of Cell Biology and GeneticsPeking UniversityBeijingPeople’s Republic of China
  3. 3.Department of Genetics, School of OncologyBeijing Institute for Cancer Research, Peking UniversityBeijingPeople’s Republic of China
  4. 4.Department of Clinical EpidemiologyThe Third Affiliated Hospital of Peking UniversityBeijingPeople’s Republic of China
  5. 5.Chinese National Human Genome CenterBeijingPeople’s Republic of China