Immunogenetics

, Volume 56, Issue 3, pp 170–177

Bioinformatic discovery and initial characterisation of nine novel antimicrobial peptide genes in the chicken

  • David J. Lynn
  • Rowan Higgs
  • Susan Gaines
  • Joanna Tierney
  • Tharappel James
  • Andrew T. Lloyd
  • Mario A. Fares
  • Grace Mulcahy
  • Cliona O’Farrelly
Original Paper

DOI: 10.1007/s00251-004-0675-0

Cite this article as:
Lynn, D.J., Higgs, R., Gaines, S. et al. Immunogenetics (2004) 56: 170. doi:10.1007/s00251-004-0675-0

Abstract

Antimicrobial peptides (AMPs) are essential components of innate immunity in a range of species from Drosophila to humans and are generally thought to act by disrupting the membrane integrity of microbes. In order to discover novel AMPs in the chicken, we have implemented a bioinformatic approach that involves the clustering of more than 420,000 chicken expressed sequence tags (ESTs). Similarity searching of proteins—predicted to be encoded by these EST clusters—for homology to known AMPs has resulted in the in silico identification of full-length sequences for seven novel gallinacins (Gal-4 to Gal-10), a novel cathelicidin and a novel liver-expressed antimicrobial peptide 2 (LEAP-2) in the chicken. Differential gene expression of these novel genes has been demonstrated across a panel of chicken tissues. An evolutionary analysis of the gallinacin family has detected sites—primarily in the mature AMP—that are under positive selection in these molecules. The functional implications of these results are discussed.

Keywords

Chicken Defensins Antimicrobial peptide Innate immune system 

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • David J. Lynn
    • 1
    • 2
  • Rowan Higgs
    • 1
    • 2
  • Susan Gaines
    • 1
  • Joanna Tierney
    • 5
  • Tharappel James
    • 1
    • 3
  • Andrew T. Lloyd
    • 1
    • 4
  • Mario A. Fares
    • 6
  • Grace Mulcahy
    • 5
  • Cliona O’Farrelly
    • 1
  1. 1.Education and Research CentreSt. Vincent’s University HospitalDublinIreland
  2. 2.Department of MedicineUniversity College DublinBelfield, DublinIreland
  3. 3.Moyne Institute of Preventive MedicineTrinity College DublinDublinIreland
  4. 4.Department of GeneticsTrinity College DublinDublinIreland
  5. 5.Department of Microbiology and Parasitology, Faculty of Veterinary MedicineUniversity College DublinBelfield, DublinIreland
  6. 6.Biology DepartmentNational University of IrelandMaynoothIreland
  7. 7.Conway InstituteUniversity College DublinBelfield, DublinIreland