European Biophysics Journal

, Volume 43, Issue 6, pp 265–276

Interceptor effect of C60 fullerene on the in vitro action of aromatic drug molecules

Authors

  • Galyna B. Skamrova
    • Department of PhysicsSevastopol National Technical University
  • Ivan Laponogov
    • Randall Division of Cell and Molecular BiophysicsNew Hunt’s House, Guy’s Campus, King’s College London
  • Anatoly S. Buchelnikov
    • Department of PhysicsSevastopol National Technical University
    • Department of Biology and ChemistryBelgorod State University
  • Yuriy G. Shckorbatov
    • Institute of Biology, V.N. Karazin Kharkiv National University
  • Svitlana V. Prylutska
    • Departments of Inorganic Chemistry and BiophysicsTaras Shevchenko National University of Kyiv
  • Uwe Ritter
    • Institute of Chemistry and BiotechnologyTechnical University of Ilmenau
  • Yuriy I. Prylutskyy
    • Departments of Inorganic Chemistry and BiophysicsTaras Shevchenko National University of Kyiv
    • Department of PhysicsSevastopol National Technical University
    • Department of Biology and ChemistryBelgorod State University
Original Paper

DOI: 10.1007/s00249-014-0960-2

Cite this article as:
Skamrova, G.B., Laponogov, I., Buchelnikov, A.S. et al. Eur Biophys J (2014) 43: 265. doi:10.1007/s00249-014-0960-2

Abstract

C60 fullerenes are spherical molecules composed purely of carbon atoms. They inspire a particularly strong scientific interest because of their specific physico-chemical properties and potential medical and nanotechnological applications. In this work we are focusing on studying the influence of the pristine C60 fullerene on biological activity of some aromatic drug molecules in human buccal epithelial cells. Assessment of the heterochromatin structure in the cell nucleus as well as the barrier function of the cell membrane was performed. The methods of cell microelectrophoresis and atomic force microscopy were also applied. A concentration-dependent restoration of the functional activity of the cellular nucleus after exposure to DNA-binding drugs (doxorubicin, proflavine and ethidium bromide) has been observed in human buccal epithelial cells upon addition of C60 fullerene at a concentration of ~10−5 M. The results were shown to follow the framework of interceptor/protector action theory, assuming that non-covalent complexation between C60 fullerene and the drugs (i.e., hetero-association) is the major process responsible for the observed biological effects. An independent confirmation of this hypothesis was obtained via investigation of the cellular response of buccal epithelium to the coadministration of the aromatic drugs and caffeine, and it is based on the well-established role of hetero-association in drug-caffeine systems. The results indicate that C60 fullerene may reverse the effects caused by the aromatic drugs, thereby pointing out the potential possibility of the use of aromatic drugs in combination with C60 fullerene for regulation of their medico-biological action.

Keywords

DNA-binding drugsC60 fullereneCaffeineHuman buccal epithelial cellsHetero-association

Supplementary material

249_2014_960_MOESM1_ESM.docx (1.5 mb)
Supplementary material 1 (DOCX 1578 kb)

Copyright information

© European Biophysical Societies' Association 2014