Review

European Biophysics Journal

, Volume 39, Issue 4, pp 551-563

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Homology modelling and spectroscopy, a never-ending love story

  • Hanka VenselaarAffiliated withCentre for Molecular and Biomolecular Informatics, CMBI, NCMLS 260, Radboud University Medical Centre Email author 
  • , Robbie P. JoostenAffiliated withCentre for Molecular and Biomolecular Informatics, CMBI, NCMLS 260, Radboud University Medical Centre
  • , Bas VrolingAffiliated withCentre for Molecular and Biomolecular Informatics, CMBI, NCMLS 260, Radboud University Medical Centre
  • , Coos A. B. BaakmanAffiliated withCentre for Molecular and Biomolecular Informatics, CMBI, NCMLS 260, Radboud University Medical Centre
  • , Maarten L. HekkelmanAffiliated withCentre for Molecular and Biomolecular Informatics, CMBI, NCMLS 260, Radboud University Medical Centre
  • , Elmar KriegerAffiliated withCentre for Molecular and Biomolecular Informatics, CMBI, NCMLS 260, Radboud University Medical Centre
  • , Gert VriendAffiliated withCentre for Molecular and Biomolecular Informatics, CMBI, NCMLS 260, Radboud University Medical Centre Email author 

Abstract

Homology modelling is normally the technique of choice when experimental structure data are not available but three-dimensional coordinates are needed, for example, to aid with detailed interpretation of results of spectroscopic studies. Herein, the state of the art of homology modelling will be described in the light of a series of recent developments, and an overview will be given of the problems and opportunities encountered in this field. The major topic, the accuracy and precision of homology models, will be discussed extensively due to its influence on the reliability of conclusions drawn from the combination of homology models and spectroscopic data. Three real-world examples will illustrate how both homology modelling and spectroscopy can be beneficial for (bio)medical research.

Keywords

Homology modelling CASP Spectroscopy