European Biophysics Journal

, Volume 38, Issue 6, pp 807–812

Structured illumination microscopy of a living cell

  • Liisa M. Hirvonen
  • Kai Wicker
  • Ondrej Mandula
  • Rainer Heintzmann
Original Paper

DOI: 10.1007/s00249-009-0501-6

Cite this article as:
Hirvonen, L.M., Wicker, K., Mandula, O. et al. Eur Biophys J (2009) 38: 807. doi:10.1007/s00249-009-0501-6

Abstract

Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.

Keywords

Structured illumination microscopyLive-cell imagingResolution improvement

Copyright information

© European Biophysical Societies' Association 2009

Authors and Affiliations

  • Liisa M. Hirvonen
    • 1
  • Kai Wicker
    • 1
  • Ondrej Mandula
    • 2
  • Rainer Heintzmann
    • 1
  1. 1.Randall Division of Cell and Molecular BiophysicsKing’s College LondonLondonUK
  2. 2.Faculty of Mathematics and PhysicsCharles UniversityPraha 2Czech Republic