European Biophysics Journal

, Volume 37, Issue 6, pp 871–877

A plate holder for non-destructive testing of mesophase crystallization assays

Authors

  • J. Agirre
    • Unidad de Biofísica (CSIC-UPV/EHU)
  • A. Mechaly
    • Unidad de Biofísica (CSIC-UPV/EHU)
  • A. Cabo-Bilbao
    • Unidad de Biofísica (CSIC-UPV/EHU)
    • CIC-Biogune Parque Tecnológico de Bizkaia Edificio 801 A
    • Unidad de Biofísica (CSIC-UPV/EHU)
    • Unidad de Biofisica (CSIC-UPV/EHU)
Original Paper

DOI: 10.1007/s00249-008-0296-x

Cite this article as:
Agirre, J., Mechaly, A., Cabo-Bilbao, A. et al. Eur Biophys J (2008) 37: 871. doi:10.1007/s00249-008-0296-x

Abstract

Here, we describe a goniometer holder to mount standard 96-well crystallization plates directly onto the goniometer head of an oscillation camera. This attachment was designed to check crystallization conditions straight from the crystallization plates under X-rays, and was proven to be useful for checking small crystals and solutions that destabilize monoolein-based lipidic cubic phase (LCP) crystallization experiments. A quick procedure for setting up LCP assays employing commercially available instruments is also reported.

Keywords

CrystallizationCubic phaseMembrane proteinsPlate holder

Supplementary material

249_2008_296_MOESM1_ESM.pdf (1.7 mb)
Lipidic cubic phase dehydration (PDF 1,702 kb)

Copyright information

© EBSA 2008