Original Paper

European Biophysics Journal

, Volume 37, Issue 6, pp 871-877

First online:

A plate holder for non-destructive testing of mesophase crystallization assays

  • J. AgirreAffiliated withUnidad de Biofísica (CSIC-UPV/EHU)
  • , A. MechalyAffiliated withUnidad de Biofísica (CSIC-UPV/EHU)
  • , A. Cabo-BilbaoAffiliated withUnidad de Biofísica (CSIC-UPV/EHU)CIC-Biogune Parque Tecnológico de Bizkaia Edificio 801 A
  • , D. M. A. GuérinAffiliated withUnidad de Biofísica (CSIC-UPV/EHU)Unidad de Biofisica (CSIC-UPV/EHU) Email author 

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Abstract

Here, we describe a goniometer holder to mount standard 96-well crystallization plates directly onto the goniometer head of an oscillation camera. This attachment was designed to check crystallization conditions straight from the crystallization plates under X-rays, and was proven to be useful for checking small crystals and solutions that destabilize monoolein-based lipidic cubic phase (LCP) crystallization experiments. A quick procedure for setting up LCP assays employing commercially available instruments is also reported.

Keywords

Crystallization Cubic phase Membrane proteins Plate holder