Original Paper

European Biophysics Journal

, Volume 36, Issue 7, pp 727-732

First online:

Atomic force microscopy images suggest aggregation mechanism in cerato-platanin

  • F. SbranaAffiliated withC.S.D.C, Dip. Fisica, Università di Firenze
  • , L. BonginiAffiliated withDip. Fisica, Università di Firenze
  • , G. CappugiAffiliated withDip. di Scienze Biochimiche, Università di Firenze
  • , D. FanelliAffiliated withC.S.D.C, Dip. Fisica, Università di FirenzeTheoretical Physics, School of Physics and Astronomy, University of Manchester, ManchesterI.N.F.N, Sezione di Firenze
  • , A. GuarinoAffiliated withUniversité de la Polynesie Francaise
  • , L. PazzagliAffiliated withDip. di Scienze Biochimiche, Università di Firenze
  • , A. ScalaAffiliated withDip. di Biotecnologie Agrarie, Sezione di Patologia Vegetale, Università di Firenze
  • , M. VassalliAffiliated withC.S.D.C, Dip. Fisica, Università di FirenzeISC, CNR, Istituto Sistemi ComplessiI.N.F.N, Sezione di Firenze
  • , C. ZoppiAffiliated withDip. di Scienze Biochimiche, Università di Firenze
    • , B. TiribilliAffiliated withC.S.D.C, Dip. Fisica, Università di FirenzeISC, CNR, Istituto Sistemi Complessi Email author 

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Abstract

Cerato-platanin (CP), the first member of the “cerato-platanin family”, is a moderately hydrophobic protein produced by Ceratocystis fimbriata, the causal agent of a severe plant disease called “canker stain”. The protein is localized in the cell wall of the fungus and it seems to be involved in the host-plane interaction and induces both cell necrosis and phytoalexin synthesis (one of the first plant defence-related events). Recently, it has been determined that CP, like other fungal surface protein, is able to self assemble in vitro. In this paper we characterize the aggregates of CP by Atomic Force Microscopy (AFM) images. We observe that CP tends to form early annular-shaped oligomers that seem to constitute the fundamental bricks of a hierarchical aggregation process, eventually resulting in large macrofibrillar assemblies. A simple model, based on the hypothesis that the aggregation is energetically favourable when the exposed surface is reduced, is compatible with the measured aggregates’ shape and size. The proposed model can help to understand the mechanism by which CP and many other fungal surface proteins exert their effects.

Keywords

Protein aggregation Cerato-platanin AFM