European Biophysics Journal

, Volume 34, Issue 8, pp 1007–1016

A simulation study on the Ca2+-independent but voltage-dependent exocytosis and endocytosis in dorsal root ganglion neurons

  • Hua Yang
  • Chen Zhang
  • Hui Zheng
  • Wei Xiong
  • Zhuan Zhou
  • Tao Xu
  • Jiu Ping Ding
Article

DOI: 10.1007/s00249-005-0486-8

Cite this article as:
Yang, H., Zhang, C., Zheng, H. et al. Eur Biophys J (2005) 34: 1007. doi:10.1007/s00249-005-0486-8

Abstract

In patch-clamped somata of dorsal root ganglion (DRG) neurons, two types of secretion have been proposed: Ca2+-dependent secretion and Ca2+-independent but voltage-dependent secretion (CIVDS). The Ca2+-induced and the depolarization-induced membrane capacitance (Cm) increases contribute 80 and 20% to the total Cm increase, respectively (Zhang and Zhou in Nat Neurosci 5:425, 2002). In order to explore the mechanism of the voltage-dependent Cm change (ΔCm), we constructed a model with sequential states. The simulation with this model closely approximates all the experimental data. The model predicts that the majority of fusion events (approximately 80%) are so-called “kiss-and-run” events, which account for the fast recovery or the rapid retrieval feature of the signals. The remaining 20% are attributed to full fusion events, which account for a slow retrieval feature. On the basis of the model, one mechanism of the activity-dependent endocytosis has revealed a differential distribution of vesicles between the kiss-and-run and full fusion states at different stimulation frequencies. The quantitative model presented in this study may help us to understand the mechanism of the CIVDS and the tightly coupled endocytosis found in mammalian DRG neurons.

Keywords

ExocytosisEndocytosisCa2+-independent but voltage-dependent secretionCapacitanceVesicle

Copyright information

© EBSA 2005

Authors and Affiliations

  • Hua Yang
    • 1
  • Chen Zhang
    • 4
  • Hui Zheng
    • 3
    • 4
  • Wei Xiong
    • 3
    • 4
  • Zhuan Zhou
    • 3
    • 4
  • Tao Xu
    • 1
    • 2
  • Jiu Ping Ding
    • 1
  1. 1.Institute of Biophysics and Biochemistry, College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanChina
  2. 2.National Laboratory of Biomacromolecules, Institute of BiophysicsChinese Academy of SciencesShanghaiChina
  3. 3.Institute of Molecular MedicinePeking UniversityBeijingChina
  4. 4.Institute of Neuroscience, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina