Article

European Biophysics Journal

, Volume 33, Issue 2, pp 117-120

Apparatus for exposing cell membranes to rapid temperature transients

  • B. SteelAffiliated withSchool of Physics, University of Sydney Email author 
  • , M. M. BilekAffiliated withSchool of Physics, University of Sydney
  • , C. G. dos RemediosAffiliated withInstitute of Biomedical Research, University of Sydney
  • , D. R. McKenzieAffiliated withSchool of Physics, University of Sydney

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Abstract

We seek to determine whether cell membranes contain sensors that trigger a downstream response to temperature excursions. To do this, we have developed a novel apparatus for exposing a cell membrane to an extremely rapid temperature excursion in the nanosecond range. Cells are plated on a gold surface that is back-heated by a pulsed laser and cooled by conduction of heat into the glass substrate and the liquid medium. Analysis using the heat diffusion equation shows that the greatest temperature rise is localized within a region tens of nanometres thick, suitable for specifically heating a cell membrane without heating the remainder of a cell. We refer to this device as a nanosecond hotplate.

Keywords

Heat diffusion equation Laser temperature jump Thermal modelling