European Biophysics Journal

, Volume 31, Issue 2, pp 118–125

Magnetophoresis and ferromagnetic resonance of magnetically labeled cells

Authors

  •  C. Wilhelm
    • Laboratoire des Milieux Désordonnés et Hétérogènes, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Case 78, 4 Place Jussieu, 75252 Paris Cedex 05, France
  •  F. Gazeau
    • Laboratoire des Milieux Désordonnés et Hétérogènes, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Case 78, 4 Place Jussieu, 75252 Paris Cedex 05, France
  •  J.-C. Bacri
    • Laboratoire des Milieux Désordonnés et Hétérogènes, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Case 78, 4 Place Jussieu, 75252 Paris Cedex 05, France
Article

DOI: 10.1007/s00249-001-0200-4

Cite this article as:
Wilhelm, C., Gazeau, F. & Bacri, J. Eur Biophys J (2002) 31: 118. doi:10.1007/s00249-001-0200-4

Abstract.

We develop in this paper two methods, based on different physical concepts, to quantify the uptake of magnetic nanoparticles in biological cells. The first one, magnetophoresis, is based on the measurement of the velocity of magnetically labeled cells submitted to a magnetic field gradient. The second one quantitates the particles' electronic spin using an electron paramagnetic resonance experiment. We show a quantitative agreement between both methods for macrophagic cells. The uptake kinetics and uptake capacity are discussed for macrophagic cells and other cell lines.

Magnetic nanoparticles Cell labeling Magnetophoresis Ferromagnetic resonance

Copyright information

© EBSA 2002