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A Metabarcoding Survey on the Fungal Microbiota Associated to the Olive Fruit Fly

  • Invertebrate Microbiology
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Abstract

The occurrence of interaction between insects and fungi is interesting from an ecological point of view, particularly when these interactions involve insect pests and plant pathogens within an agroecosystem. In this study, we aimed to perform an accurate analysis on the fungal microbiota associated to Bactrocera oleae (Rossi) through a metabarcoding approach based on 454 pyrosequencing. From this analysis, we retrieved 43,549 reads that clustered into 128 operational taxonomic units (OTUs), of which 29 resulted in the “core” associate fungi of B. oleae. This fungal community was mainly represented by sooty mould fungi, such as Cladosporium spp., Alternaria spp. and Aureobasidium spp., by plant pathogens like Colletotrichum spp. and Pseudocercospora spp., along with several other less abundant taxa whose ecology is unclear in most of the cases. Our findings lead to new insights into the microbial ecology of this specific ecological niche, enabling the understanding of a complex network of interactions within the olive agroecosystem.

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Acknowledgments

This work was partially funded by SAF@MED (PON a3_00016)-PON Ricerca e competitività 2007-2013 and PON PON03PE_00090_2 “Modelli sostenibili e nuove tecnologie per la valorizzazione delle olive e dell’olio extravergine di oliva prodotto in Calabria”-PON Ricerca e competitività 2007–2013.

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Authors and Affiliations

  1. Dipartimento di Agraria, Università degli Studi “Mediterranea” di Reggio Calabria, Reggio Calabria, Italy

    Antonino Malacrinò, Leonardo Schena, Orlando Campolo, Francesca Laudani, Saveria Mosca, Giulia Giunti, Cinzia Patricia Strano & Vincenzo Palmeri

  2. Dipartimento di Scienze Agrarie e Forestali, Università degli Studi di Palermo, Palermo, Italy

    Antonino Malacrinò

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  1. Antonino Malacrinò
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  8. Vincenzo Palmeri
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Correspondence to Leonardo Schena.

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Conflict of Interest

The authors declare that they have no conflicts of interest.

Authors’ Contribution

All authors designed the research. LS and VP supervised and coordinated the work. AM, FL, SM and OC provided samples. AM performed lab work, data analysis and data interpretation. AM wrote the first draft of the manuscript. All authors contributed to, corrected and approved the final manuscript.

Data Accessibility

Raw data from 454 sequencing are available on NCBI SRA database under the Project Accession ID: SRP089714. Sequences used for phylogenetic analysis are available as Supplementary material 2.

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Malacrinò, A., Schena, L., Campolo, O. et al. A Metabarcoding Survey on the Fungal Microbiota Associated to the Olive Fruit Fly. Microb Ecol 73, 677–684 (2017). https://doi.org/10.1007/s00248-016-0864-z

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  • DOI: https://doi.org/10.1007/s00248-016-0864-z

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