Microbial Ecology

, Volume 64, Issue 2, pp 346–358

Mesocosms of Aquatic Bacterial Communities from the Cuatro Cienegas Basin (Mexico): A Tool to Test Bacterial Community Response to Environmental Stress

  • Silvia Pajares
  • German Bonilla-Rosso
  • Michael Travisano
  • Luis E. Eguiarte
  • Valeria Souza
Microbiology of Aquatic Systems

DOI: 10.1007/s00248-012-0045-7

Cite this article as:
Pajares, S., Bonilla-Rosso, G., Travisano, M. et al. Microb Ecol (2012) 64: 346. doi:10.1007/s00248-012-0045-7

Abstract

Microbial communities are responsible for important ecosystem processes, and their activities are regulated by environmental factors such as temperature and solar ultraviolet radiation. Here we investigate changes in aquatic microbial community structure, diversity, and evenness in response to changes in temperature and UV radiation. For this purpose, 15 mesocosms were seeded with both microbial mat communities and plankton from natural pools within the Cuatro Cienegas Basin (Mexico). Clone libraries (16S rRNA) were obtained from water samples at the beginning and at the end of the experiment (40 days). Phylogenetic analysis indicated substantial changes in aquatic community composition and structure in response to temperature and UV radiation. Extreme treatments with elevation in temperature or UV radiation reduced diversity in relation to the Control treatments, causing a reduction in richness and increase in dominance, with a proliferation of a few resistant operational taxonomic units. Each phylum was affected differentially by the new conditions, which translates in a differential modification of ecosystem functioning. This suggests that the impact of environmental stress, at least at short term, will reshape the aquatic bacterial communities of this unique ecosystem. This work also demonstrates the possibility of designing manageable synthetic microbial community ecosystems where controlled environmental variables can be manipulated. Therefore, microbial model systems offer a complementary approach to field and laboratory studies of global research problems associated with the environment.

Supplementary material

248_2012_45_MOESM1_ESM.doc (50 kb)
Table S1(DOC 50 kb)
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Table S2(DOC 43 kb)
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Table S5(DOC 69 kb)
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Figure S1(PDF 345 kb)
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Figure S2(PDF 1634 kb)

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Silvia Pajares
    • 1
    • 2
  • German Bonilla-Rosso
    • 1
  • Michael Travisano
    • 3
  • Luis E. Eguiarte
    • 1
  • Valeria Souza
    • 1
  1. 1.Departamento de Ecología Evolutiva, Instituto de EcologíaUniversidad Nacional Autónoma de México (UNAM)Mexico CityMexico
  2. 2.Consejo Superior de Investigaciones Científicas (IRNA-CSIC)SalamancaSpain
  3. 3.Department of Ecology, Evolution and BehaviorUniversity of MinnesotaSaint PaulUSA