Microbial Ecology

, Volume 64, Issue 1, pp 268–278

Gut-Associated Bacteria Throughout the Life Cycle of the Bark Beetle Dendroctonus rhizophagus Thomas and Bright (Curculionidae: Scolytinae) and Their Cellulolytic Activities

  • Jesús Morales-Jiménez
  • Gerardo Zúñiga
  • Hugo C. Ramírez-Saad
  • César Hernández-Rodríguez
INVERTEBRATE MICROBIOLOGY

DOI: 10.1007/s00248-011-9999-0

Cite this article as:
Morales-Jiménez, J., Zúñiga, G., Ramírez-Saad, H.C. et al. Microb Ecol (2012) 64: 268. doi:10.1007/s00248-011-9999-0
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Abstract

Dendroctonus rhizophagus Thomas and Bright (Curculionidae: Scolytinae) is an endemic economically important insect of the Sierra Madre Occidental in Mexico. This bark beetle has an atypical behavior within the genus because just one beetle couple colonizes and kills seedlings and young trees of 11 pine species. In this work, the bacteria associated with the Dendroctonus rhizophagus gut were analyzed by culture-dependent and culture-independent methods. Analysis of 16S rRNA sequences amplified directly from isolates of gut bacteria suggests that the bacterial community associated with Dendroctonus rhizophagus, like that of other Dendroctonus spp. and Ips pini, is limited in number. Nine bacterial genera of γ-Proteobacteria and Actinobacteria classes were detected in the gut of Dendroctonus rhizophagus. Stenotrophomonas and Rahnella genera were the most frequently found bacteria from Dendroctonus rhizophagus gut throughout their life cycle. Stenotrophomonas maltophilia, Ponticoccus gilvus, and Kocuria marina showed cellulolytic activity in vitro. Stenotrophomonas maltophilia, Rahnella aquatilis, Raoultella terrigena, Ponticoccus gilvus, and Kocuria marina associated with larvae or adults of Dendroctonus rhizophagus could be implicated in nitrogen fixation and cellulose breakdown, important roles associated to insect development and fitness, especially under the particularly difficult life conditions of this beetle.

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jesús Morales-Jiménez
    • 1
    • 3
  • Gerardo Zúñiga
    • 2
  • Hugo C. Ramírez-Saad
    • 3
  • César Hernández-Rodríguez
    • 1
  1. 1.Departamento de Microbiología, Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalDistrito FederalMexico
  2. 2.Departamento de Zoología, Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalDistrito FederalMexico
  3. 3.Laboratorio de Ecología Molecular, Departamento de Sistemas BiológicosUniversidad Autónoma Metropolitana—XochimilcoMéxicoMexico