Genes and Genomes

Microbial Ecology

, Volume 61, Issue 1, pp 123-133

First online:

Assessing Gut Microbial Diversity from Feces and Rectal Mucosa

  • Ana DurbánAffiliated withCentro Superior de Investigación en Salud Pública (CSISP)Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de ValènciaCIBER en Epidemiología y Salud Pública (CIBEResp)
  • , Juan J. AbellánAffiliated withCentro Superior de Investigación en Salud Pública (CSISP)Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de ValènciaCIBER en Epidemiología y Salud Pública (CIBEResp)
  • , Nuria Jiménez-HernándezAffiliated withCentro Superior de Investigación en Salud Pública (CSISP)Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de ValènciaCIBER en Epidemiología y Salud Pública (CIBEResp)
  • , Marta PonceAffiliated withServicio de Medicina Digestiva, Hospital Universitario La FeCentro de Investigación Biomédica en Enfermedades Hepáticas y Digestivas (CIBER-EHD)
  • , Julio PonceAffiliated withServicio de Medicina Digestiva, Hospital Universitario La FeCentro de Investigación Biomédica en Enfermedades Hepáticas y Digestivas (CIBER-EHD)
  • , Teresa SalaAffiliated withServicio de Medicina Digestiva, Hospital Universitario La FeCentro de Investigación Biomédica en Enfermedades Hepáticas y Digestivas (CIBER-EHD)
  • , Giuseppe D’AuriaAffiliated withCentro Superior de Investigación en Salud Pública (CSISP)Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de ValènciaCIBER en Epidemiología y Salud Pública (CIBEResp)
  • , Amparo LatorreAffiliated withCentro Superior de Investigación en Salud Pública (CSISP)Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de ValènciaCIBER en Epidemiología y Salud Pública (CIBEResp)
  • , Andrés MoyaAffiliated withCentro Superior de Investigación en Salud Pública (CSISP)Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de ValènciaCIBER en Epidemiología y Salud Pública (CIBEResp) Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Gut microbiota is the most complex bacterial community in the human body and its study may give important clues to the etiology of different intestinal diseases. Most studies carried out so far have used fecal samples, assuming that these samples have a similar distribution to the communities present throughout the colon. The present study was designed to test this assumption by comparing samples from the rectal mucosa and feces of nine healthy volunteers by sequencing libraries of 16S rRNA genes. At the family taxonomic level, where rarefaction curves indicate that the observed number of taxa is close to the expected one, we observe under different statistical analyses that fecal and mucosal samples cluster separately. The same is found at the level of species considering phylogenetic information. Consequently, it cannot be stated that both samples from a given individual are of similar composition. We believe that the evidence in support of this statement is strong and that it would not change by increasing the number of individuals and/or performing massive sequencing. We do not expect clinicians to stop using feces for research, but we think it is important to caution them on their potential lack of representativeness with respect to the bacterial biofilm on the rectal mucosa.