Microbial Ecology

, Volume 59, Issue 1, pp 187–198

In Vitro and In Vivo Evaluation of the Prebiotics GroBiotic®-A, Inulin, Mannanoligosaccharide, and Galactooligosaccharide on the Digestive Microbiota and Performance of Hybrid Striped Bass (Morone chrysops × Morone saxatilis)

  • Gary Burr
  • Michael Hume
  • Steven Ricke
  • David Nisbet
  • Delbert GatlinIII
Host Microbe Interactions

DOI: 10.1007/s00248-009-9597-6

Cite this article as:
Burr, G., Hume, M., Ricke, S. et al. Microb Ecol (2010) 59: 187. doi:10.1007/s00248-009-9597-6

Abstract

Two separate experiments were conducted with hybrid striped bass to evaluate four potential prebiotics: GroBiotic®-A (partially autolyzed brewer’s yeast, dairy ingredient components, and fermentation products), mannanoligosaccharide (MOS), galactooligosaccharide (GOS), and inulin. In the in vitro experiment, intestinal contents were incubated with the individual prebiotics (0.5% by weight) at 25°C for 24 and 48 h. Analysis of volatile fatty acids in the supernatant showed that GroBiotic®-A, MOS, and GOS tended to produce lower acetate levels but higher butyrate levels at 48 h compared to diet alone. However, denaturing gradient gel electrophoresis (DGGE) analysis failed to detect any differences in the composition of the microbial community among treatments. DNA sequencing of a common band for all inoculated samples revealed close similarity to the anaerobic Fusobacteria bacterium. An 8-week feeding trial also was conducted to evaluate the four prebiotics looking at growth performance; weight gain, feed efficiency ratio, protein efficiency ratio, whole-body ash, moisture, and lipid did not vary among fish fed the various diets. However, DGGE analysis revealed that all prebiotics produced a different type of microbial community in the intestinal tract of hybrid striped bass compared to fish fed the basal diet. Thus, GroBiotic®-A, FOS, GOS, and MOS exhibited prebiotic effects in hybrid striped bass.

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Gary Burr
    • 1
  • Michael Hume
    • 2
    • 3
  • Steven Ricke
    • 4
  • David Nisbet
    • 2
  • Delbert GatlinIII
    • 1
  1. 1.Department of Wildlife and Fisheries SciencesTexas A&M UniversityCollege StationUSA
  2. 2.Food and Feed Safety UnitUSDA, Agricultural Research ServiceCollege StationUSA
  3. 3.Department of Poultry ScienceTexas A&M UniversityCollege StationUSA
  4. 4.Center for Food Safety, ISFE and Food Science DepartmentUniversity of ArkansasFayettevilleUSA