Microbial Ecology

, Volume 59, Issue 1, pp 158–173

Species Composition of Bacterial Communities Influences Attraction of Mosquitoes to Experimental Plant Infusions

Authors

  • Loganathan Ponnusamy
    • Department of EntomologyNorth Carolina State University
  • Dawn M. Wesson
    • Department of Tropical MedicineTulane University
  • Consuelo Arellano
    • Department of StatisticsNorth Carolina State University
  • Coby Schal
    • Department of EntomologyNorth Carolina State University
    • Department of EntomologyNorth Carolina State University
Microbiology of Aquatic Systems

DOI: 10.1007/s00248-009-9565-1

Cite this article as:
Ponnusamy, L., Wesson, D.M., Arellano, C. et al. Microb Ecol (2010) 59: 158. doi:10.1007/s00248-009-9565-1

Abstract

In the container habitats of immature mosquitoes, catabolism of plant matter and other organic detritus by microbial organisms produces metabolites that mediate the oviposition behavior of Aedes aegypti and Aedes albopictus. Public health agencies commonly use oviposition traps containing plant infusions for monitoring populations of these mosquito species, which are global vectors of dengue viruses. In laboratory experiments, gravid females exhibited significantly diminished responses to experimental infusions made with sterilized white oak leaves, showing that attractive odorants were produced through microbial metabolic activity. We evaluated effects of infusion concentration and fermentation time on attraction of gravid females to infusions made from senescent bamboo or white oak leaves. We used plate counts of heterotrophic bacteria, total counts of 4′,6-diamidino-2-phenylindole-stained bacterial cells, and 16S ribosomal DNA (rDNA) polymerase chain reaction–denaturing gradient gel electrophoresis (DGGE) to show that changes in the relative abundance of bacteria and the species composition of bacterial communities influenced attraction of gravid A. aegypti and A. albopictus mosquitoes to infusions. DGGE profiles showed that bacterial species composition in infusions changed over time. Principal components analysis indicated that oviposition responses to plant infusions were in general most affected by bacterial diversity and abundance. Analysis of bacterial 16S rDNA sequences derived from DGGE bands revealed that Proteobacteria (Alpha-, Beta-, Delta-, and Gamma-) were the predominant bacteria detected in both types of plant infusions. Gravid A. aegypti were significantly attracted to a mix of 14 bacterial species cultured from bamboo leaf infusion. The oviposition response of gravid mosquitoes to plant infusions is strongly influenced by abundance and diversity of bacterial species, which in turn is affected by plant species, leaf biomass, and fermentation time.

Supplementary material

248_2009_9565_MOESM1_ESM.doc (50 kb)
Supplemental Table 1Bacterial phylotypes identified* from predominant 16S rDNA-DGGE bands from BL infusions. Phylogenetic affiliations were determined based on classification of band sequences in a neighbor-joining phylogenetic tree (DOC 50 kb)
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Supplemental Table 2Bacterial phylotypes identified* from predominant 16S rDNA-DGGE bands from WOL infusions. Phylogenetic affiliations were determined based on classification of band sequences in a neighbor-joining phylogenetic tree (DOC 60 kb)
248_2009_9565_MOESM3_ESM.doc (38 kb)
Supplemental Table 3Results of mixed-model ANOVA of effects of trial and fermentation time (day) on bacterial cell growth and diversity in BL infusions (DOC 38 kb)
248_2009_9565_MOESM4_ESM.doc (38 kb)
Supplemental Table 4Effects of infusion concentration§ and fermentation time on bacterial species diversity in WOL infusions (DOC 37 kb)
248_2009_9565_MOESM5_ESM.doc (30 kb)
Supplemental Table 5Correlation matrix for infusion-associated variables used in principal components analysis (DOC 29.5 kb)

Copyright information

© Springer Science+Business Media, LLC 2009