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Influence of Hydrological Pulse on Bacterial Growth and DOC Uptake in a Clear-Water Amazonian Lake

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Abstract

This study was conducted to evaluate: (1) the bacterial growth and the dissolved organic carbon (DOC) uptake in an Amazonian lake (Lake Batata) at high-water and low-water periods of the flood pulse; (2) the influence of nitrogen and phosphorus (NP) additions on bacterial growth and DOC uptake in Lake Batata at two flood pulse periods; and (3) the bioavailability of the main DOC sources in Lake Batata. Lake Batata is a typical clear-water Amazonian lake, located in the watershed of Trombetas River, Central Amazon, Brazil. Bacterial batch cultures were set up with 90% 0.2-μm filtered water and 10% inoculum from Lake Batata. N-NH4NO3 and P-KH2PO4, with final concentrations of 50 and 5 μM, respectively, were added to the cultures, except for controls. Extra sources of DOC (e.g., algal lysate, plant leachates) were added to constitute six distinct treatments. Bacterial response was measured by maximum bacterial abundance and rates of bacterial production, respiration, DOC uptake, and bacterial growth efficiency (BGE). Bacterial growth and DOC uptake were higher in NP treatments than in controls, indicating a consistent nutrient limitation in Lake Batata. The composition of DOC also seems to be an important regulating factor of bacterial growth in Lake Batata. Seasonally, bacterial growth and DOC bioavailability were higher at low-water period, when the phytoplankton is a significant extra source of DOC, than at high-water period, when the forest is the main source of DOC. DOC bioavailability was better estimated based on the diversity and the diagenetic stage of carbon compounds than on single classes of labile compounds. Changes in BGE were better related to CNP stoichiometry in the water, and the “excess” of organic substrates was oxidized in catabolism, despite the quality of these compounds for bacterial growth. Finally, we conclude that bacterial growth and DOC uptake vary throughout the flood pulse in clear-water Amazonian ecosystems as a result of changes in nutrient concentration and in DOC composition.

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Acknowledgments

The authors are particularly grateful to A.M. Amado and Dr. A.S. Furtado for criticism of the manuscript, to Dr. D. Giroldo for carbohydrate analyses, and to Dra. D.S.D. Araújo for correcting the English. CNPq and Mineração Rio do Norte supported this research. V.F. Farjalla is particularly grateful to FAPERJ Institution for a Ph.D. scholarship.

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Authors and Affiliations

  1. Lab. Limnologia, Dept. de Ecologia, Inst. de Biologia, CCS-UFRJ, PO Box 68020, Rio de Janeiro, RJ, 21941-590, Brazil

    Vinicius F. Farjalla, Francisco A. Esteves, Reinaldo L. Bozelli & Alex Enrich-Prast

  2. Dept. de Química Orgânica, Inst. de Química, CT-UFRJ, Rio de Janeiro, RJ, 21949-900, Brazil

    Debora A. Azevedo

  3. Dept. de Biologia Geral, UFJF, Juiz de Fora, MG, 36036-330, Brazil

    Fabio Roland

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  1. Vinicius F. Farjalla
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Correspondence to Vinicius F. Farjalla.

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Farjalla, V.F., Azevedo, D.A., Esteves, F.A. et al. Influence of Hydrological Pulse on Bacterial Growth and DOC Uptake in a Clear-Water Amazonian Lake. Microb Ecol 52, 334–344 (2006). https://doi.org/10.1007/s00248-006-9021-4

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