Microbial Ecology

, Volume 47, Issue 1, pp 104–113

The Relationship between Microbial Community Structure and Functional Stability, Tested Experimentally in an Upland Pasture Soil

  • B. S. Griffiths
  • H. L. Kuan
  • K. Ritz
  • L. A. Glover
  • A. E. McCaig
  • C. Fenwick
Article

DOI: 10.1007/s00248-002-2043-7

Cite this article as:
Griffiths, B., Kuan, H., Ritz, K. et al. Microb Ecol (2004) 47: 104. doi:10.1007/s00248-002-2043-7

Abstract

Soil collected from an upland pasture was manipulated experimentally in ways shown previously to alter microbial community structure. One set of soil was subjected to chloroform fumigation for 0, 0.5, 2, or 24 h and the other was sterilised by gamma-irradiation and inoculated with a 10−2, 10−4, 10−6, or 10−8 dilution of a soil suspension prepared from unsterilized soil. Following incubation for 8 months, to allow for the stabilization of microbial biomass and activity, the resulting microbial community structure (determined by PCR-DGGE of bacterial specific amplification products of total soil DNA) was assessed. In addition, the functional stability (defined here as the resistance and resilience of short-term decomposition of plant residues to a transient heat or a persistent copper perturbation) was determined. Changes in the active bacterial population following perturbation (determined by RT-PCR-DGGE of total soil RNA) were also monitored. The manipulations resulted in distinct shifts in microbial community structure as shown by PCR-DGGE profiles, but no significant decreases in the number of bands. These shifts in microbial community structure were associated with a reduction in functional stability. The clear correlation between altered microbial community structure and functional stability observed in this upland pasture soil was not evident when the same protocols were applied to soils in other studies. RT-PCR-DGGE profiles only detected a shift in the active bacterial population following heat, but not copper, perturbation. We conclude that the functional stability of decomposition is related to specific components of the microbial community.

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • B. S. Griffiths
    • 1
  • H. L. Kuan
    • 1
  • K. Ritz
    • 2
  • L. A. Glover
    • 3
  • A. E. McCaig
    • 3
    • 4
  • C. Fenwick
    • 3
    • 5
  1. 1.Plant–Soil Interface ProgrammeScottish Crop Research Institute, Invergowrie, Dundee DD2 5DAUK
  2. 2.National Soil Resources InstituteCranfield University, Silsoe, Bedfordshire MK45 4DTUK
  3. 3.Department of Molecular and Cell Biology, Institute of Medical SciencesUniversity of Aberdeen, Aberdeen AB25 2ZDUK
  4. 4.The Engineering and Physical Sciences Research Council, Polaris House, North Star Avenue, Swindon SN2 1ETUK
  5. 5.The Natural Environment Research Council, Polaris House, North Star Avenue, Swindon SN2 1EUUK