Diffusion imaging and tractography of congenital brain malformations
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- Wahl, M., Barkovich, A.J. & Mukherjee, P. Pediatr Radiol (2010) 40: 59. doi:10.1007/s00247-009-1448-6
Diffusion imaging is an MRI modality that measures the microscopic molecular motion of water in order to investigate white matter microstructure. The modality has been used extensively in recent years to investigate the neuroanatomical basis of congenital brain malformations. We review the basic principles of diffusion imaging and of specific techniques, including diffusion tensor imaging (DTI) and high angular resolution diffusion imaging (HARDI). We show how DTI and HARDI, and their application to fiber tractography, has elucidated the aberrant connectivity underlying a number of congenital brain malformations. Finally, we discuss potential uses for diffusion imaging of developmental disorders in the clinical and research realms.
KeywordsDiffusion tensor DTI White matter Brain malformations Children
Diffusion MRI has quickly become an important part of neuroradiology research, finding application in essentially every disorder involving white matter, from schizophrenia to multiple sclerosis to traumatic brain injury. Within the field of pediatric radiology, diffusion imaging has allowed researchers to make discoveries about the neuroanatomical features underlying several congenital disorders.
This review will briefly summarize the theory behind diffusion MR imaging and discuss newer techniques for more advanced white matter microstructural analysis and fiber tractography, including diffusion tensor imaging (DTI) and high angular resolution diffusion imaging (HARDI). We explore the application of diffusion imaging and fiber tracking to four congenital brain malformations: horizontal gaze palsy with progressive scoliosis (HGPPS), pontine tegmental cap dysplasia (PTCD), holoprosencephaly, and agenesis of the corpus callosum (AgCC). Finally we discuss potential applications of diffusion imaging in clinical neuroradiology. This discussion is not intended to be an exhaustive review of the DTI literature on congenital malformations. Rather, we show in depth how these new imaging technologies have contributed knowledge for a few conditions, in order to highlight the uses of diffusion imaging in research and clinical settings.
Theory and acquisition
Large white matter tracts are composed of numerous axons organized in a parallel fashion. Water contained within these structures diffuses through random molecular motion (Brownian motion), but such motion is constrained by densely packed axonal membranes and myelin sheaths. Thus water will preferentially diffuse along the direction of the axon bundle rather than perpendicular to it. The measurement of this anisotropic diffusion of water forms the basis for diffusion imaging [1, 2].
Diffusion imaging is performed by adding a pair of diffusion-sensitizing spatial gradient pulses to a T2-weighted spin-echo MR sequence. The gradients are applied in the same direction before and after the 180° refocusing RF pulse, a sequence called Stejskal-Tanner diffusion encoding . Thus molecular motion of water along the spatial gradient causes an incomplete rephasing of proton spins and resultant loss of signal intensity. Whole-brain images are acquired with the gradient applied in a number of independent directions; a reference image with no applied gradient is also acquired. Information from all images can then be combined to evaluate the three-dimensional profile of diffusion in each voxel. Key acquisition parameters include the b factor, which depends on the strength, duration, and timing of gradient pulses, and the number of diffusion-encoding directions acquired. Typical b values used in the clinical setting range from 600 s/mm2 to 1,500 s/mm2, though high angular resolution techniques (discussed below) use higher values .
Diffusion tensor imaging
Diffusion tensor imaging approximates the diffusion profile in each voxel as a three-dimensional Gaussian function, most commonly visualized as an ellipsoid. Mathematically, this approximation is represented by a symmetric 3 × 3 matrix with six independent elements (the “diffusion tensor”) . DTI thus requires diffusion measurements in at least six non-collinear directions, although more are frequently used to improve image quality. From the diffusion tensor, a number of useful calculations can be performed. First, the three eigenvectors and eigenvalues of the matrix can be calculated. These correspond to the directions and lengths of the three principal axes of the ellipsoid. The eigenvector corresponding to the largest eigenvalue represents the major axis, and thus (in brain imaging) the predominant direction of axons within a given voxel.
Also, the proportion of diffusion along the major axis relative to the minor axes is calculated as the fractional anisotropy (FA) and represents a quantitative measure of white matter microstructural integrity. While the precise biological factors that contribute to FA are unclear, it is thought of as a composite measure of axonal density, directional coherence of axon bundles, and degree of myelination . FA values take a range from 0 (representing isotropic diffusion) to 1 (representing diffusion entirely restricted to one direction). FA has been found to be a highly reproducible measurement in healthy subjects  and has been shown to correlate with cognitive performance on a variety of measures in both adult [7, 8, 9] and pediatric populations [10, 11, 12].
The direction of the primary eigenvector is commonly combined with FA measurements on DTI colormaps, in which the color of each voxel represents the principal diffusion direction, and the brightness represents FA. These colormaps provide a visual representation of the size and orientation of various white-matter structures and are useful for both diagnostic and research purposes. DTI tractography can also be performed in which directional information from neighboring voxels is combined to estimate the three-dimensional structure of major white-matter pathways [13, 14, 15]. In congenital malformations, tractography can be used to characterize dysplastic white-matter tracts or to identify ectopic fibers. In addition, FA measurements can be averaged over an entire white-matter tract, yielding a composite quantitative measure of tract organization. A recent two-part review of DTI provides additional details on its theory and application, including recommended acquisition parameters for both 1.5-T and 3-T systems [4, 16].
High angular resolution diffusion imaging
A major disadvantage of DTI is its inability to recover more than one fiber orientation within a single voxel. Thus, in regions of complex white matter architecture, where fibers merge or cross, DTI is unable to accurately depict white matter microstructure. To overcome this limitation, HARDI combines stronger diffusion-weighting (higher b values) with diffusion measurements in a larger number of independent directions (on the order of 50 or more) to generate a high angular resolution measurement of three-dimensional (3-D) diffusion . HARDI measurements yield a more complete description of the 3-D diffusion profile than the diffusion tensor and thus facilitate the reconstruction of multiple fiber directions within a single voxel. Q-ball (QBI) imaging is one example of a HARDI reconstruction technique that has yielded detailed information about complex white matter structure within a clinically feasible scan time [18, 19, 20]. HARDI methods have not been extensively used to investigate pediatric conditions but are likely to find increasing use in the future.
Applications to congenital malformations
Horizontal gaze palsy with progressive scoliosis
Horizontal gaze palsy with progressive scoliosis (HGPPS) is a congenital malformation limited to the hindbrain and spine. It is a rare autosomal-recessive condition characterized by congenital absence of normal horizontal gaze, as well as scoliosis that progresses through childhood and adolescence. No other neurological or behavioral abnormalities have been associated with the condition. The causative gene has been identified as ROBO3, which encodes a receptor important for axon guidance across the midline . Both structural and diffusion imaging have been performed on individuals with HGPPS.
DTI investigations have greatly advanced our understanding of the anatomy underlying HGPPS. One study examined the DTI color images of a patient with HGPPS to find the absence of normal decussating fibers in the pons, including superior cerebellar peduncles and pontocerebellar fibers (Fig. 1) . Another study performed DTI tractography to reveal exclusively ipsilateral ascending and descending connectivity through the lower brainstem, as well as ipsilaterally projecting tracts from primary motor areas . The corpus callosum was found to be grossly normal, suggesting a midline-crossing defect localized to the brainstem. This study also made functional MRI measurements while the patient performed a motor activity, which revealed only ipsilateral motor and sensory cortical activation. These DTI studies, together with the anatomic findings of deep midline clefts in the brainstem, suggest that a general defect in hindbrain midline axon crossing underlies HGPPS. These findings are in excellent agreement with the known function of the causative gene (ROBO3) in guiding midline axon crossing. Thus DTI analysis, along with genetic studies, has successfully elucidated the molecular and neuroanatomical basis of HGPPS.
Pontine tegmental cap dysplasia
The mechanisms underlying pontine tegmental cap dysplasia (PTCD) have been less fully elucidated. Clinical findings in PTCD are more varied and include multiple cranial neuropathies (acoustic nerve palsy in all patients with variable involvement of facial and trigeminal nerves), impaired swallowing, and cerebellar and pyramidal motor symptoms . Global developmental delay is commonly noted, suggesting supratentorial involvement. The genetic basis for PTCD has not been found; analysis of axonal guidance genes NTN1 and DCC failed to identify any pathogenic mutations .
Diffusion imaging has helped point toward the pathologic basis for PTCD. Tractography studies have revealed the absence of superior and middle cerebellar peduncle decussation, as well as an ectopic fiber tract that crosses the midline on the dorsal aspect of the pons, forming the characteristic tegmental cap (Fig. 2) [27, 28]. This ectopic fiber tract finding points to a specific pathology of either neuronal migration or axon guidance of pontine gray neurons originating from the rhombic lip and has generated specific hypotheses to explain its presence (Fig. 2) . Possibilities include insufficient or aberrant neuronal migration away from the rhombic lip with normal subsequent axonal guidance toward the contralateral MCP, or normal neuronal migration with aberrant axon guidance away from the ventral surface. These processes are mediated by multiple guidance molecules and suggest candidate genes that might be responsible for the condition. Although the causative gene has not been identified, DTI findings have narrowed the focus of the search.
Holoprosencephaly is a congenital disorder of forebrain development characterized by the partial or complete failure of induction of the anterior ventral forebrain with resultant absence of the anterior ventromedial cerebrum and absent anterior interhemispheric fissure. It is usually classified, from least to most severe, as lobar, semilobar or alobar. Clinical characteristics include mental retardation, facial dysmorphism, spastic quadriparesis, athetoid movements, epilepsy and endocrine disorders . Conventional MR imaging studies have focused on further characterizing the condition based on morphology of the corpus callosum , basal ganglia  and sulcal patterns . The last study  found the angle between sylvian fissures (the “sylvian angle”) in the two hemispheres to be a reliable marker for severity of holoprosencephaly.
Agenesis of the corpus callosum
Agenesis of the corpus callosum (AgCC) is a congenital brain malformation with a frequency of about 1 in 4,000 individuals. It is characterized by the partial or complete absence of callosal fibers, and accompanied by a spectrum of neuropsychological deficits, including many falling within the autistic spectrum . Interestingly, the symptoms associated with AgCC are not replicated by surgical transection of the callosum, and interhemispheric integration of information is relatively intact in most AgCC individuals . Thus the symptoms seen with AgCC cannot be explained by callosal absence alone. Much imaging research into AgCC has focused on identifying and characterizing other possible anatomic features that contribute to the phenotype.
Studies using conventional MR imaging have focused on identifying and classifying brain malformations associated with AgCC. One study found polymicrogyria (PMG) and cortical and subcortical heterotopias to be common . However, many people with AgCC have no associated cortical malformations yet show similar symptoms to those who do. While conventional MR imaging remains useful for diagnosing AgCC and cataloging other anatomic features that are evident clinically, it remains an inadequate technique for probing the basic pathobiology behind AgCC.
Recent preliminary work using Q-ball imaging and tractography suggests that a significant number of fibers in the Probst bundles are not in fact corticocortical association fibers but instead project to subcortical regions, including thalamus and brainstem (unpublished data). This observation has an interesting correspondence in a mouse knockout model of AgCC, in which the Satb2 gene is disrupted, causing callosal axons to be reprogrammed to project to ipsilateral subcortical regions . This correspondence might point to specific candidate genes for AgCC. Whether this initial finding of aberrant subcortical connectivity in AgCC is verified remains to be seen, but it highlights the power of diffusion tractographic studies over conventional imaging: while conventional imaging provides useful clinical anatomic markers, diffusion imaging studies of white-matter connectivity enable investigators to probe the basic underlying developmental pathobiology that gave rise to the condition.
Additional diffusion-imaging investigations have focused on individuals with partial callosal agenesis (pAgCC). Subjects with pAgCC retain a residual callosal fragment but are symptomatically similar to those with complete AgCC . There are examples of individuals with partial and complete agenesis within a single family, suggesting that the developmental and genetic causes of the two conditions are similar. Based on conventional imaging studies alone, it is unclear whether the callosal fragments are simply a result of the arrested growth of normal callosal projections or they are formed in a more complex manner.
Two diffusion imaging studies have examined the callosal connectivity in pAgCC in some detail. The first study used DTI tractography to examine five patients with pAgCC . Within these patients, the study found a relatively consistent pattern of anteriorly located callosal fragments with primarily homotopic frontal connectivity. The study also revealed an “asymmetric sigmoid bundle” that coursed from right anterior frontal lobe to left occipitotemporal lobes. This heterotopic callosal fiber is not observed in normal individuals and suggests that pAgCC is formed through a more complicated process than arrested callosal development.
A more recent study used high-resolution Q-ball imaging and tractography to map the callosal connectivity in pAgCC in more detail . The study, with six patients, found considerable variability in both the position and the connectivity of callosal fragments (Fig. 4). Crucially, the cortical connectivity through a given fragment could not be predicted by its position alone; fragments similar in size and position were found to have vastly different connectivity patterns. In addition, a number of heterotopic callosal connections were found between varied cortical areas, extending the asymmetric sigmoid bundle finding of the previous study. These studies indicate that the process of development of callosal fragments in pAgCC is a complex and plastic process, distinct from normal callosal development. It is possible that the same signaling mechanisms that yield aberrant intrahemispheric connectivity (through the Probst bundles) also give rise to novel interhemispheric connectivity patterns through the callosal fragment.
A final study examined the microstructural organization of the cingulum bundle in subjects with AgCC . Because of the observed clinical deficits in executive function and social–emotional processing that map to the anterior cingulate cortex, it was hypothesized that individuals with AgCC had reduced organization of the ventral cingulum bundle. DTI tractography was performed in 12 AgCC patients along with 12 matched controls, and the FA of the ventral cingulum bundle was measured. T1-weighted structural images were also used to measure the tract volume. Compared with controls, the study revealed that AgCC patients demonstrated a reduced FA specific to the right cingulum bundle, along with bilateral reductions in tract volume. Thus, in addition to the presence of aberrant connections, individuals with AgCC appear to have alterations to the organization of normal ipsilateral white-matter tracts, which might contribute significantly to the behavioral phenotype. AgCC might be a more widespread white-matter disorder than previously appreciated.
Summary of imaging and genetic findings. For each of the four congenital malformations discussed, major conventional MRI and diffusion imaging findings are listed, along with genetic findings associated with the condition
1. Depressed floor of 4th ventricle
1. Absence of decussating SCP and pontocerebellar fibers
ROBO3 mutation 
2. Hypoplastic pons and medulla, with “butterfly medulla” sign
2. Ipsilaterally projecting corticospinal tracts
1. Hypoplastic ventral pons
1. Absence of SCP and MCP decussations
Unknown; no mutations observed in NTN1 or DCC 
2. Dorsal pontine cap projecting into 4th ventricle
2. Ectopic dorsal pontine decussating tract
1. Absent ventromedial cerebrum and anterior hemispheric fissure
1. CSTs absent in alobar HPE
Multiple associated genes 
2. CST and MCP size correlate with cognitive performance
3. Multiple altered supratentorial white matter tracts in semilobar HPE
1. Callosal absence, with or without associated cortical malformations
1. Heterotopic tracts in partial AgCC
Multiple associated genes 
2. Probst bundles
2. Reduced FA in VCB
3. Reduced VCB tract volume
The same diffusion imaging studies that have advanced our knowledge of congenital brain malformations point to potential clinical uses. In the hindbrain malformations PTCD and HGPPS, while conventional imaging findings can suggest the diagnosis, the DTI findings of defective brainstem midline decussation (HGPPS) and ectopic dorsal transverse pontine fibers (PTCD) are more specific signs. In holoprosencephaly, the size of corticospinal tracts and middle cerebellar peduncles on DTI images correlates well with assessments of neurodevelopment and might be utilized for prognostic purposes.
In studies of AgCC, considerable variation has been found in the connectivity of Probst bundles and, in the case of partial agenesis, callosal fragments. As more patients are examined with diffusion tractography, these variations might be found to correlate with behavioral and cognitive performance. Such findings would be very useful clinically, leading to early prognosis of developmental outcome. Finally, quantitative FA measurements of the cingulum bundle, and likely other white matter structures, represent potentially useful biomarkers for the severity of the condition.
Diffusion imaging has also been applied to more common pediatric conditions that typically do not reveal findings on conventional neuroimaging, such as autism [44, 45, 46, 47, 48, 49, 50]. White matter alterations in these neurodevelopmental disorders have been found to be more widespread. As these findings are increasingly correlated with cognitive measures, diffusion imaging might inform cognitive and behavioral therapy on the basis of FA measurements in specific white-matter regions. Also, the combination of quantitative imaging findings with genetic analyses might isolate genes involved in polygenic conditions . Because diffusion imaging provides uniquely detailed quantitative information about white-matter microstructural organization and connectivity, its applications to pediatric neuroradiology are likely to proliferate.
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