Urological Research

, Volume 26, Issue 6, pp 383–388

Proteins in renal stones and urine of stone formers

  • Anwar A. Siddiqui
  • Taranum Sultana
  • Niels-Peter Buchholz
  • Mohammad A. Waqar
  • Jamsheer Talati
ORIGINAL PAPER

DOI: 10.1007/s002400050073

Cite this article as:
Siddiqui, A., Sultana, T., Buchholz, N. et al. Urological Research (1998) 26: 383. doi:10.1007/s002400050073

Abstract

Knowledge of the essential characteristics of macromolecules constituting the organic matrix of the nidus of urinary stones is required to understand the mechanism of urolithogenesis. The aim of this study was to isolate and characterise those stone nidus proteins. Using an extraction buffer containing SDS and β-mercaptoethanol, we were able to overcome known problems of protein isolation from urinary stone matrix. These proteins were characterised by a strong tendency to aggregate under reducing and denaturing conditions. On SDS-PAGE, their molecular weights range from ≤12 to 66 kDa. Antisera raised against stone matrix proteins showed a cross-reactivity between proteins isolated from different stones irrespective of their origin or mineral composition. Moreover, urinary proteins from stone formers also cross-reacted with these whereas there was no reaction with urinary proteins of non-stone formers. Western blotting confirmed these findings. Given the above summarised properties, it can be safely concluded that these proteins are prevalent in urines of stone formers, that they are selectively incorporated into renal stones of all aetiologies, and that they most likely have a role in nidus and, therefore, early stone formation.

Key words UrolithiasisUrinary proteinsStone matrix proteinsLow molecular weight proteinsSDS-PAGE

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Anwar A. Siddiqui
    • 1
  • Taranum Sultana
    • 1
  • Niels-Peter Buchholz
    • 2
  • Mohammad A. Waqar
    • 1
  • Jamsheer Talati
    • 2
  1. 1.Department of Biochemistry, The Aga Khan University, P.O. Box 3500, Karachi 74800, Pakistan E-mail: anwar.siddiqui@aku.edu; Fax: 92 21 493 42 94PK
  2. 2.Department of Surgery (Urology) The Aga Khan University, Karachi, PakistanPK