Journal of Molecular Evolution

, Volume 72, Issue 4, pp 339–351

Novel Hydrophobins from Trichoderma Define a New Hydrophobin Subclass: Protein Properties, Evolution, Regulation and Processing

  • Verena Seidl-Seiboth
  • Sabine Gruber
  • Ugur Sezerman
  • Torsten Schwecke
  • Aydin Albayrak
  • Torsten Neuhof
  • Hans von Döhren
  • Scott E. Baker
  • Christian P. Kubicek
Article

DOI: 10.1007/s00239-011-9438-3

Cite this article as:
Seidl-Seiboth, V., Gruber, S., Sezerman, U. et al. J Mol Evol (2011) 72: 339. doi:10.1007/s00239-011-9438-3

Abstract

Hydrophobins are small proteins, characterised by the presence of eight positionally conserved cysteine residues, and are present in all filamentous asco- and basidiomycetes. They are found on the outer surfaces of cell walls of hyphae and conidia, where they mediate interactions between the fungus and the environment. Hydrophobins are conventionally grouped into two classes (class I and II) according to their solubility in solvents, hydropathy profiles and spacing between the conserved cysteines. Here we describe a novel set of hydrophobins from Trichoderma spp. that deviate from this classification in their hydropathy, cysteine spacing and protein surface pattern. Phylogenetic analysis shows that they form separate clades within ascomycete class I hydrophobins. Using T. atroviride as a model, the novel hydrophobins were found to be expressed under conditions of glucose limitation and to be regulated by differential splicing.

Keywords

HydrophobinTrichodermaProtein evolutionProtein processingPeptidomicsSplicingHypocrea

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Verena Seidl-Seiboth
    • 1
  • Sabine Gruber
    • 1
  • Ugur Sezerman
    • 2
  • Torsten Schwecke
    • 3
    • 4
  • Aydin Albayrak
    • 2
  • Torsten Neuhof
    • 3
  • Hans von Döhren
    • 4
  • Scott E. Baker
    • 5
  • Christian P. Kubicek
    • 1
  1. 1.Research Area Gene Technology and Applied Biochemistry, Institute of Chemical EngineeringVienna University of TechnologyViennaAustria
  2. 2.Biological Sciences and BioengineeringSabanci UniversityIstanbulTurkey
  3. 3.Anagnostec GmbH, Im Biotechnologiepark TGZ IILuckenwaldeGermany
  4. 4.TU Berlin, Institut für ChemieFG Biochemie und Molekulare BiologieBerlinGermany
  5. 5.Fungal Biotechnology Team, Chemical and Biological Process Development GroupPacific Northwest National LaboratoryRichlandUSA