Journal of Molecular Evolution

, Volume 60, Issue 5, pp 615–634

Structure, Evolution, and Expression of the Two Invertase Gene Families of Rice

  • Xuemei Ji
  • Wim Van den Ende
  • Andre Van Laere
  • Shihua Cheng
  • John Bennett
Article

DOI: 10.1007/s00239-004-0242-1

Cite this article as:
Ji, X., Van den Ende, W., Van Laere, A. et al. J Mol Evol (2005) 60: 615. doi:10.1007/s00239-004-0242-1

Abstract

Invertases catalyze the irreversible hydrolysis of sucrose to glucose and fructose. Plants contain two unrelated families of these enzymes: acid forms that derive from periplasmic invertases of eubacteria and are found in cell wall and vacuole, and neutral/alkaline forms evolved from the cytosolic invertases of cyanobacteria. Genomes of rice (Oryza sativa) and thale cress (Arabidopsis thaliana) contain multiple genes encoding these two families. Here for rice we identify the member genes of a cell-wall group (designated OsCIN19), a vacuolar group (OsVIN12), and two ancient neutral/alkaline groups: α (OsNIN14) and β (OsNIN58). In Arabidopsis these groups contain six, two, four and five members, respectively. It is believed that the vacuolar group evolved from the cell-wall group. We provide evidence that the N-terminal signal peptide that directs cell-wall invertases co-translationally into the endoplasmic reticulum for secretion was replaced in the vacuolar group by a sequence similar to the complex N-terminal motif that targets alkaline phosphatase post-translationally to the vacuolar membrane of yeast. Since the last common ancestor of Arabidopsis and rice, the two invertase families evolved equally rapidly via gene duplication and gene loss, but the acid invertase family underwent ∼10 events of intron loss compared with a single event of intron gain in the neutral/alkaline invertase family. Transcripts were detected for all rice invertase genes except OsCIN9. The acid invertase genes showed greater spatial and temporal diversity of expression than the neutral/alkaline genes.

Keywords

Cell wall Vacuole Cytosol Mitochondrion Chloroplast Oryza sativa Arabidopsis thaliana Subcellular targeting Intron loss 

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Xuemei Ji
    • 1
    • 2
  • Wim Van den Ende
    • 3
  • Andre Van Laere
    • 3
  • Shihua Cheng
    • 2
  • John Bennett
    • 1
  1. 1.Plant Breeding, Genetics and Biochemistry DivisionInternational Rice Research Institute, DAPOMetro ManilaPhilippines
  2. 2.Chinese National Rice Research InstituteZhejiangChina
  3. 3.Laboratory of Molecular Plant Physiology, Institute of Botany and MicrobiologyKatholieke Universiteit LeuvenLeuvenBelgium

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