Original Paper

European Journal of Plastic Surgery

, Volume 34, Issue 5, pp 351-358

First online:

Application of regulated oxygen-enriched negative pressure-assisted wound therapy in combating anaerobic infections

Oxygen-enriched negative pressure treatment
  • Moris TopazAffiliated withPlastic Surgery Unit, Hillel Yaffe Medical CenterDepartment of Chemistry, Bar-Ilan University Email author 
  • , Orly BiskerAffiliated withSackler Medical School, Tel-Aviv University
  • , Moni LitmanovitchAffiliated withGeneral Intensive Care Unit, Hillel Yaffe Medical Center
  • , Gershon KerenAffiliated withInfectious Diseases Unit, Hillel Yaffe Medical Center

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Regulated negative pressure-assisted wound therapy (RNPT) applies non-pharmaceutical technology for enhanced healing of hard to cure, acute and chronic wounds. Although used for over two decades, wound pO2 levels, an essential physical parameter inherent in RNPT that follows Henry’s Law of gases, have not been reported. Necrotizing fasciitis (NF), a severe soft tissue infection, necessitates prompt intensive therapeutic response using pharmaceutical, surgical, and hyperbaric oxygen treatment when anaerobes are involved. We report a linear decrease in pO2 values in direct correlation with sub-atmospheric pressure and concurrent changes in wound atmospheric pO2 by supplemented oxygen in an in vitro phantom leg simulated wound model treated with RNPT. We designed a system using regulated oxygen-enriched negative pressure therapy (RO-NPT) for controlling wound atmospheric pO2. A representative patient case report treated for NF with RO-NPT is presented. RNPT follows Henry’s law of gases and leads to a decrease in wound atmospheric pO2. The application of RNPT in anaerobic wound infections should be contraindicated. Wound pO2 is enhanced by simultaneous application of oxygen by RO-NPT. We have demonstrated the rationale for the possible use of RO-NPT for prevention of anaerobic wound infections and as a supplemental mode of treatment of NF.


RNPT RO-NPT Henry’s law Wound infection Anaerobic infection Necrotizing fasciitis HBO