Article

The Journal of Membrane Biology

, Volume 247, Issue 9, pp 815-826

Functionalized Amphipols: A Versatile Toolbox Suitable for Applications of Membrane Proteins in Synthetic Biology

  • Eduardo Antonio Della PiaAffiliated withBio-Nanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-Science Center, University of Copenhagen
  • , Randi Westh HansenAffiliated withBio-Nanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-Science Center, University of Copenhagen
  • , Manuela ZoonensAffiliated withInstitut de Biologie Physico-Chimique, UMR 7099, CNRS/Université Paris-7
  • , Karen L. MartinezAffiliated withBio-Nanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-Science Center, University of Copenhagen Email author 

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Abstract

Amphipols are amphipathic polymers that stabilize membrane proteins isolated from their native membrane. They have been functionalized with various chemical groups in the past years for protein labeling and protein immobilization. This large toolbox of functionalized amphipols combined with their interesting physico-chemical properties give opportunities to selectively add multiple functionalities to membrane proteins and to tune them according to the needs. This unique combination of properties makes them one of the most versatile strategies available today for exploiting membrane proteins onto surfaces for various applications in synthetic biology. This review summarizes the properties of functionalized amphipols suitable for synthetic biology approaches.

Keywords

Biosensors Self-assembly Protein immobilization Protein labeling Multiple functionalization