Expression, Purification and Functional Reconstitution of Slack Sodium-Activated Potassium Channels
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- Yan, Y., Yang, Y., Bian, S. et al. J Membrane Biol (2012) 245: 667. doi:10.1007/s00232-012-9425-7
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The slack (slo2.2) gene codes for a potassium-channel α-subunit of the 6TM voltage-gated channel family. Expression of slack results in Na+-activated potassium channel activity in various cell types. We describe the purification and reconstitution of Slack protein and show that the Slack α-subunit alone is sufficient for potassium channel activity activated by sodium ions as assayed in planar bilayer membranes and in membrane vesicles.