The Journal of Membrane Biology

, Volume 245, Issue 11, pp 667–674

Expression, Purification and Functional Reconstitution of Slack Sodium-Activated Potassium Channels

  • Yangyang Yan
  • Youshan Yang
  • Shumin Bian
  • Fred J. Sigworth
Article

DOI: 10.1007/s00232-012-9425-7

Cite this article as:
Yan, Y., Yang, Y., Bian, S. et al. J Membrane Biol (2012) 245: 667. doi:10.1007/s00232-012-9425-7

Abstract

The slack (slo2.2) gene codes for a potassium-channel α-subunit of the 6TM voltage-gated channel family. Expression of slack results in Na+-activated potassium channel activity in various cell types. We describe the purification and reconstitution of Slack protein and show that the Slack α-subunit alone is sufficient for potassium channel activity activated by sodium ions as assayed in planar bilayer membranes and in membrane vesicles.

Keywords

Artificial planar membrane Ion channel expression and reconstitution Patch clamp Potassium ion channel 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Yangyang Yan
    • 1
  • Youshan Yang
    • 1
  • Shumin Bian
    • 1
  • Fred J. Sigworth
    • 1
  1. 1.Department of Cellular and Molecular PhysiologyYale UniversityNew HavenUSA