Journal of Membrane Biology

, Volume 233, Issue 1, pp 105–118

Melatonin Reduces Apoptosis Induced by Calcium Signaling in Human Leukocytes: Evidence for the Involvement of Mitochondria and Bax Activation

  • Javier Espino
  • Ignacio Bejarano
  • Pedro C. Redondo
  • Juan A. Rosado
  • Carmen Barriga
  • Russel J. Reiter
  • José A. Pariente
  • Ana B. Rodríguez
Article

DOI: 10.1007/s00232-010-9230-0

Cite this article as:
Espino, J., Bejarano, I., Redondo, P.C. et al. J Membrane Biol (2010) 233: 105. doi:10.1007/s00232-010-9230-0

Abstract

We have evaluated the effect of melatonin on apoptosis evoked by increases in [Ca2+]c in human leukocytes. Our results show that treatment of neutrophils with the calcium mobilizing agonist FMLP or the specific inhibitor of calcium reuptake thapsigargin induced a transient increase in [Ca2+]c. Our results also show that FMLP and thapsigargin increased caspase-9 and -3 activities and the active forms of both caspases. The effect of FMLP and thapsigargin on caspase activation was time-dependent. Similar results were obtained when lymphocytes were stimulated with thapsigargin. This stimulatory effect was accompanied by induction of mPTP, activation of the proapoptotic protein Bax and release of cytochrome c. However, when leukocytes were pretreated with melatonin, all of the apoptotic features indicated above were significantly reversed. Our results suggest that melatonin reduces caspase-9 and -3 activities induced by increases in [Ca2+]c in human leukocytes, which are produced through the inhibition of both mPTP and Bax activation.

Keywords

Melatonin Caspases Cytochrome c Mitochondria Apoptosis Leukocytes 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Javier Espino
    • 1
  • Ignacio Bejarano
    • 1
  • Pedro C. Redondo
    • 1
  • Juan A. Rosado
    • 1
  • Carmen Barriga
    • 1
  • Russel J. Reiter
    • 2
  • José A. Pariente
    • 1
  • Ana B. Rodríguez
    • 1
  1. 1.Department of PhysiologyUniversity of ExtremaduraBadajozSpain
  2. 2.Department of Cellular and Structural BiologyThe University of Texas Health Sciences CenterSan AntonioUSA

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