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Comprehensive Analyses of Transport Proteins Encoded Within the Genome of “Aromatoleum aromaticum” Strain EbN1

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Abstract

The denitrifying bacterium “Aromatoleum aromaticum” strain EbN1 is specialized for the aerobic utilization of aromatic compounds including crude oil constituents. We here report whole-genome analyses for potential transport proteins in A. aromaticum strain EbN1. This organism encodes very few transporters for simple sugars and most other common carbon sources. However, up to 28% of its putative transporters may act on fairly hydrophobic aromatic and aliphatic compounds. We categorize the putative transporters encoded within the genome, assign them to recognized families, and propose their preferred substrates. The bioinformatic data are correlated with available metabolic information to obtain an integrated view of the metabolic network of A. aromaticum strain EbN1. The results thus indicate that this organism possesses a disproportionately large percentage of transporters for the uptake and efflux of hydrophobic and amphipathic aromatic and aliphatic compounds compared with previously analyzed organisms. We predict that these findings will have important implications for our ecophysiological understanding of bioremediation.

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Acknowledgments

This work was supported by NIH Grant GM077402 from the National Institute of General Medical Sciences. We thank Mary Beth Hiller, Van T. Duong, and Jeeni Criscenzo for assistance in the preparation of the manuscript.

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Authors and Affiliations

  1. Division of Biological Sciences, University of California at San Diego, La Jolla, CA, 92093-0116, USA

    Dorjee G. Tamang & Milton H. Saier Jr.

  2. Max-Planck-Institute for Marine Microbiology, Celsiusstr. 1, 28359, Bremen, Germany

    Ralf Rabus

  3. Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl-von-Ossietzky University Oldenburg, 26111, Oldenburg, Germany

    Ralf Rabus

  4. Department of Plant Sciences, University of California at Davis, One Shield Avenue, Davis, CA, 95616, USA

    Ravi D. Barabote

Authors
  1. Dorjee G. Tamang
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  2. Ralf Rabus
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  3. Ravi D. Barabote
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  4. Milton H. Saier Jr.
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Correspondence to Milton H. Saier Jr..

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Tamang, D.G., Rabus, R., Barabote, R.D. et al. Comprehensive Analyses of Transport Proteins Encoded Within the Genome of “Aromatoleum aromaticum” Strain EbN1. J Membrane Biol 229, 53–90 (2009). https://doi.org/10.1007/s00232-009-9168-2

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  • DOI: https://doi.org/10.1007/s00232-009-9168-2

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