Marine Biology

, 163:79

Use of near-infrared reflectance spectroscopy to quantify diet mixing in a generalist marine herbivore

Original paper

DOI: 10.1007/s00227-016-2852-8

Cite this article as:
Bain, K.F. & Poore, A.G.B. Mar Biol (2016) 163: 79. doi:10.1007/s00227-016-2852-8

Abstract

The diet of individual animals in the field is governed by behavioural preference as well as factors intrinsic and extrinsic to the organism that may constrain their ability to obtain preferred foods. Accurate measurements of individual diets are essential to understanding how consumers can impact communities, but can be difficult to obtain. We aim to determine whether near-infrared reflectance spectroscopy (NIRS) can be used to quantify the degree of diet mixing in the diet of a generalist herbivore, the marine gastropod Lunella torquatus. NIRS successfully classified five algal diets and could quantify the proportions of these species in two-species combinations. We then assessed whether NIRS could predict dietary composition post-digestion by contrasting faecal material from single-species and mixed diets. Partial least squares methods were used to develop prediction models that effectively discriminated among species and, for most species, effectively predicted the proportion of a given species in all possible two-species mixtures. NIRS thus has the potential to provide a non-invasive method for assessing the realised diets of free-living herbivores.

Supplementary material

227_2016_2852_MOESM1_ESM.pdf (572 kb)
Supplementary material 1 (PDF 572 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Evolution and Ecology Research Centre, School of Biological and Earth and Environmental ScienceUniversity of New South WalesSydneyAustralia