Calcified Tissue International

, Volume 63, Issue 3, pp 230–235

Alendronate Reduces Adhesion of Human Osteoclast-like Cells to Bone and Bone Protein-Coated Surfaces

  • S.  Colucci
  • V.  Minielli
  • G.  Zambonin
  • N.  Cirulli
  • G.  Mori
  • M.  Serra
  • V.  Patella
  • A.  Zambonin Zallone
  • M.  Grano
Article

DOI: 10.1007/s002239900519

Cite this article as:
Colucci, S., Minielli, V., Zambonin, G. et al. Calcif Tissue Int (1998) 63: 230. doi:10.1007/s002239900519

Abstract

Bisphosphonates (BPs) are potent inhibitors of bone resorption and are therapeutically effective in disease of increased bone turnover, but their mechanism(s) of action remain to be elucidated. Using as experimental model human osteoclast-like cell lines derived from giant cell tumors of bone, extensively characterized for their osteoclast features, we investigated the adhesive properties of osteoclasts on bone slices and on different proteins of the extracellular matrix in the presence of BPs. Adhesion assays using bone slices pretreated with ALN, at the established active concentration, showed that, although the morphology of osteoclasts plated onto pretreated bone slices was not modified, the number of adherent cells was reduced by the treatment of about 50% vs. controls. The effect of ALN on the adhesion of osteoclast-like cells onto specific extracellular matrix proteins, such as bone sialoprotein-derived peptide, containing the RGD sequence, conjugated to BSA (BSP-BSA) and fibronectin (FN), was also tested. In the case of FN the treatment with ALN of protein-coated wells did not modify the percentage of cell adhesion compared with the control, whereas onto BSP-BSA the presence of ALN significantly reduced adhesion of about 40–45%, suggesting that the inhibitory effect of ALN on cell adhesion could probably be due to the interference with receptors specifically recognizing bone matrix proteins as αVβ3 integrins. Furthermore, ALN induced Ca-mediated intracellular signals in osteoclasts, triggering a 2-fold increase in intracellular calcium concentration.

Key words: Alendronate—Bone proteins—Osteoclasts.

Copyright information

© Springer-Verlag New York 1998

Authors and Affiliations

  • S.  Colucci
    • 1
  • V.  Minielli
    • 1
  • G.  Zambonin
    • 2
  • N.  Cirulli
    • 1
  • G.  Mori
    • 1
  • M.  Serra
    • 3
  • V.  Patella
    • 2
  • A.  Zambonin Zallone
    • 1
  • M.  Grano
    • 1
  1. 1.Istituto di Anatomia Umana Normale P.zza G. CesarBariItaly
  2. 2.I Clinica Ortopedica Università di Bari,BariItaly
  3. 3.Laboratorio di Ricerca OncologicaBolognaItaly