Calcified Tissue International

, Volume 87, Issue 3, pp 226–235

Association of Oxidative Stress with Postmenopausal Osteoporosis and the Effects of Hydrogen Peroxide on Osteoclast Formation in Human Bone Marrow Cell Cultures

  • Ki Hyun Baek
  • Ki Won Oh
  • Won Young Lee
  • Seong Su Lee
  • Mee Kyoung Kim
  • Hyuk Sang Kwon
  • Eun Jung Rhee
  • Je Ho Han
  • Ki Ho Song
  • Bong Yun Cha
  • Kwang Woo Lee
  • Moo Il Kang
Article

DOI: 10.1007/s00223-010-9393-9

Cite this article as:
Baek, K.H., Oh, K.W., Lee, W.Y. et al. Calcif Tissue Int (2010) 87: 226. doi:10.1007/s00223-010-9393-9

Abstract

It has been suggested that oxidative stress is associated with the pathogenesis of osteoporosis. The objective of this study was to explore the association between a marker of oxidative stress and either bone turnover markers or bone mineral density (BMD) in postmenopausal women. In addition, the effects of oxidative stress on the formation of osteoclasts in human bone marrow cell culture were examined. We performed a cross-sectional analysis in healthy postmenopausal women aged 60–78 years (n = 135, 68.2 ± 4.9). Oxidative stress was evaluated in the serum by measuring 8-hydroxy-2′-deoxyguanosine (8-OH-dG) levels. The biochemical markers of bone turnover and areal BMD were measured in all participants. Multivariate linear regression analysis revealed a negative association between 8-OH-dG levels and BMD of the lumbar spine, total hip, femoral neck, and trochanter and positive association with type I collagen C-telopeptide (ICTP) levels. The odds ratio of 8-OH-dG for osteoporosis was 1.54 (1.14–2.31, P = 0.003). In cultures of primary human marrow cells, H2O2 caused concentration-dependent activation of TRAP-positive multinucleated giant cells. H2O2 also increased the area of pits per osteoclast activity assay substrate. RT-PCR showed that H2O2 stimulated the expression of M-CSF and RANKL and increased the RANKL/OPG ratio. The data support the view that oxidative stress is associated with increased bone resorption and low bone mass in otherwise healthy women. In addition, RANKL and M-CSF stimulation induced by oxidative stress may participate in osteoclastogenesis in human bone.

Keywords

Oxidative stressOsteoporosis8-Hydroxy-2′-deoxyguanosineBone turnover markerBone mineral densityOsteoclast

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Ki Hyun Baek
    • 1
  • Ki Won Oh
    • 2
  • Won Young Lee
    • 2
  • Seong Su Lee
    • 1
  • Mee Kyoung Kim
    • 1
  • Hyuk Sang Kwon
    • 1
  • Eun Jung Rhee
    • 2
  • Je Ho Han
    • 1
  • Ki Ho Song
    • 1
  • Bong Yun Cha
    • 1
  • Kwang Woo Lee
    • 1
  • Moo Il Kang
    • 1
    • 3
  1. 1.Department of Internal Medicine, College of MedicineThe Catholic University of KoreaSeoulKorea
  2. 2.Department of Internal MedicineSungkyunkwan University School of MedicineSeoulKorea
  3. 3.Division of Endocrinology and Metabolism, Department of Internal Medicine, Seoul St. Mary’s HospitalThe Catholic University of KoreaSeocho-Gu SeoulKorea