Calcified Tissue International

, Volume 85, Issue 2, pp 119–126

Lysyl Oxidase (Lox) Gene Deficiency Affects Osteoblastic Phenotype

  • N. Pischon
  • J. M. Mäki
  • P. Weisshaupt
  • N. Heng
  • A. H. Palamakumbura
  • P. N’Guessan
  • A. Ding
  • R. Radlanski
  • H. Renz
  • T. A. L. J. J. Bronckers
  • J. Myllyharju
  • A. M. Kielbassa
  • B. M. Kleber
  • J.-P. Bernimoulin
  • P. C. Trackman
Article

DOI: 10.1007/s00223-009-9252-8

Cite this article as:
Pischon, N., Mäki, J.M., Weisshaupt, P. et al. Calcif Tissue Int (2009) 85: 119. doi:10.1007/s00223-009-9252-8

Abstract

Lysyl oxidase (LOX) catalyzes cross-linking of elastin and collagen, which is essential for the structural integrity and function of bone tissue. The present study examined the role of Lox gene deficiency for the osteoblast phenotype in primary calvarial osteoblasts from E18.5 Lox knockout (Lox−/−) and wild type (wt) (C57BL/6) mice. Next to Lox gene depletion, mRNA expression of Lox isoforms, LOXL1–4, was significantly downregulated in Lox−/− bone tissue. A significant decrease of DNA synthesis of Lox−/− osteoblasts compared to wt was found. Early stages of osteoblastic apoptosis studied by annexin-V binding as well as later stages of DNA fragmentation were not affected. However, mineral nodule formation and osteoblastic differentiation were markedly decreased, as revealed by significant downregulation of osteoblastic markers, type I collagen, bone sialoprotein, and Runx2/Cbfa1.

Keywords

Lysyl oxidaseLOXL1–4KnockoutOsteoblast

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • N. Pischon
    • 1
  • J. M. Mäki
    • 2
  • P. Weisshaupt
    • 1
  • N. Heng
    • 1
  • A. H. Palamakumbura
    • 3
  • P. N’Guessan
    • 4
  • A. Ding
    • 5
  • R. Radlanski
    • 5
  • H. Renz
    • 5
  • T. A. L. J. J. Bronckers
    • 6
  • J. Myllyharju
    • 2
  • A. M. Kielbassa
    • 1
  • B. M. Kleber
    • 1
  • J.-P. Bernimoulin
    • 1
  • P. C. Trackman
    • 3
  1. 1.Department of Operative Dentistry and Periodontology, CharitéCentrum 3, University School of Dental MedicineCharité – Universitätsmedizin BerlinBerlinGermany
  2. 2.Oulu Center for Cell-Matrix Research, Biocenter Oulu, Department of Medical Biochemistry and Molecular BiologyUniversity of OuluOuluFinland
  3. 3.Department of Oral Biology and Periodontology, Goldman School of Dental MedicineBoston UniversityBostonUSA
  4. 4.Department of Infectious Diseases and PulmonologyCharité – UniversitätsmedizinBerlinGermany
  5. 5.Department of Experimental Dental MedicineCharité – UniversitätsmedizinBerlinGermany
  6. 6.Department of Oral Cell Biology, ACTAVrije UniversiteitAmsterdamThe Netherlands