Calcified Tissue International

, Volume 83, Issue 2, pp 146–154

A Comparison of the Physical and Chemical Differences Between Cancellous and Cortical Bovine Bone Mineral at Two Ages

  • Liisa T. Kuhn
  • Marc D. Grynpas
  • Christian C. Rey
  • Yaotang Wu
  • Jerome L. Ackerman
  • Melvin J. Glimcher
Article

DOI: 10.1007/s00223-008-9164-z

Cite this article as:
Kuhn, L.T., Grynpas, M.D., Rey, C.C. et al. Calcif Tissue Int (2008) 83: 146. doi:10.1007/s00223-008-9164-z

Abstract

To assess possible differences between the mineral phases of cortical and cancellous bone, the structure and composition of isolated bovine mineral crystals from young (1–3 months) and old (4–5 years) postnatal bovine animals were analyzed by a variety of complementary techniques: chemical analyses, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and 31P solid-state magic angle spinning nuclear magnetic resonance spectroscopy (NMR). This combination of methods represents the most complete physicochemical characterization of cancellous and cortical bone mineral completed thus far. Spectra obtained from XRD, FTIR, and 31P NMR all confirmed that the mineral was calcium phosphate in the form of carbonated apatite; however, a crystal maturation process was evident between the young and old and between cancellous and cortical mineral crystals. Two-way analyses of variance showed larger increases of crystal size and Ca/P ratio for the cortical vs. cancellous bone of 1–3 month than the 4–5 year animals. The Ca/(P + CO3) remained nearly constant within a given bone type and in both bone types at 4–5 years. The carbonate and phosphate FTIR band ratios revealed a decrease of labile ions with age and in cortical, relative to cancellous, bone. Overall, the same aging or maturation trends were observed for young vs. old and cancellous vs. cortical. Based on the larger proportion of newly formed bone in cancellous bone relative to cortical bone, the major differences between the cancellous and cortical mineral crystals must be ascribed to differences in average age of the crystals.

Keywords

Bone mineral Cancellous Cortical Trabecular Aging Crystallinity Fourier transform infrared spectroscopy X-ray diffraction Solid-state nuclear magnetic resonance 

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Liisa T. Kuhn
    • 1
    • 2
  • Marc D. Grynpas
    • 3
  • Christian C. Rey
    • 4
  • Yaotang Wu
    • 1
    • 5
  • Jerome L. Ackerman
    • 1
    • 5
  • Melvin J. Glimcher
    • 1
  1. 1.Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedic SurgeryChildren’s Hospital, Harvard Medical SchoolBostonUSA
  2. 2.Department of Reconstructive SciencesUniversity of Connecticut Health CenterFarmingtonUSA
  3. 3.Samuel Lunenfeld Research InstituteMt. Sinai HospitalTorontoCanada
  4. 4.Laboratoire de Physico-Chimie des SolidesCentre National de la Recherche ScientifiqueToulouseFrance
  5. 5.Biomaterials Laboratory, Martinos Center, Department of RadiologyMassachusetts General HospitalCharlestownUSA