Experimental Brain Research

, 199:411

Theta burst and conventional low-frequency rTMS differentially affect GABAergic neurotransmission in the rat cortex

Authors

  • Jörn Trippe
    • Department of Neurophysiology, Medical FacultyRuhr-University Bochum
  • Annika Mix
    • Department of Neurophysiology, Medical FacultyRuhr-University Bochum
  • Selcen Aydin-Abidin
    • Department of Neurophysiology, Medical FacultyRuhr-University Bochum
    • Department of Neurophysiology, Medical FacultyRuhr-University Bochum
  • Alia Benali
    • Department of Neurophysiology, Medical FacultyRuhr-University Bochum
Research Article

DOI: 10.1007/s00221-009-1961-8

Cite this article as:
Trippe, J., Mix, A., Aydin-Abidin, S. et al. Exp Brain Res (2009) 199: 411. doi:10.1007/s00221-009-1961-8

Abstract

Modified cortical excitability following repetitive transcranial magnetic stimulation (rTMS) may be related to short- or long-term synaptic plasticity of neuronal excitation but could also affect cortical inhibition. Therefore, in the rat we tested how three different rTMS protocols, intermittent and continuous theta-burst (iTBS, cTBS), and low-frequency 1 Hz stimulation, change the expression of GAD65, GAD67 and GAT-1 which are expressed in cortical inhibitory interneurons in an activity-dependent manner. Acutely (2 h), all protocols reduced the expression of GAD67 in frontal, motor, somatosensory and visual cortex but increased that of GAD65 and GAT-1 to different degree, with iTBS having the strongest acute effect. The initial decrease in GAD67 reversed after 1 day, leading to a strong increase in GAD67 expression for up to 7 days primarily in the frontal cortex in case of iTBS, cTBS and in all studied areas following 1 Hz rTMS. While also GAD65 and GAT-1 expression reversed after 1 day in case of iTBS and cTBS, 1 Hz rTMS induced a steady increase in GAD65 and GAT-1 expression during the 7 days investigated. Our data demonstrate that rTMS affects the expression of activity-dependent proteins of the cortical inhibitory interneurons. Besides common effects of low- (1 Hz) and high-frequency (TBS) stimulation on protein expression, differences in quantity and time course of changes point to differences in the contribution of possible neuronal subsystems. Further studies are needed to distinguish cell-type specific effects.

Keywords

Repetitive TMSTheta-burst stimulationGAD67GAD65GAT-1Cortical inhibitionSynaptic plasticity

Copyright information

© Springer-Verlag 2009