Experimental Brain Research

, Volume 183, Issue 4, pp 477–485

Changes of metabolite profile in kainic acid induced hippocampal injury in rats measured by HRMAS NMR

  • Hui Mao
  • Donna Toufexis
  • Xiaoxia Wang
  • Agnès Lacreuse
  • Shaoxiong Wu
Research Article

DOI: 10.1007/s00221-007-1061-6

Cite this article as:
Mao, H., Toufexis, D., Wang, X. et al. Exp Brain Res (2007) 183: 477. doi:10.1007/s00221-007-1061-6

Abstract

The solid-state high resolution magic angle spinning nuclear magnetic resonance (HRMAS NMR) technique was applied in this work to characterize and quantify the neurochemical changes in the rat hippocampus (CA1 or CA3) after local administration of kainic acid (KA). Intact tissue samples obtained from the KA treated and control brain samples were analyzed using HRMAS NMR. Metabolite profiles from NMR spectra of KA treated and control samples revealed the statistical significant decrease of N-acetylaspartate (NAA) and an increase of choline derivatives in the CA1 and CA3 directly receiving KA injection. Less extensive KA-induced metabolic changes were found in the hippocampi sample from the area contralateral to the site receiving KA administration. These results provided quantitative metabolic information on KA-induced neuronal loss and cell breakdown. In addition, the present study also revealed increased level of γ-aminobutyric acid (GABA) and glutamate after KA treatment, suggesting that the cellular release of inhibitory and excitatory amino acids can be quantified using this method. KA induced microglia activation was evidenced by increased level of myo-insitol (myo-I). This study demonstrates that ex vivo HRMAS NMR is a useful tool for analyzing and quantifying changes of neurochemistry and cerebral metabolism in the intact brain.

Keywords

Neurochemistry Cerebral metabolism Kainic acid NMR Hippocampus Neurotransmitter Brain injury 

Abbreviations

KA

Kainic acid

HRMAS

High resolution magic angle spinning

NMR

Nuclear magnetic resonance

MRS

Magnetic resonance spectroscopy

NAA

N-acetyl-aspartate

Cho

Choline

PC

Phosphocholine

GPC

Glyocerphosphocholine

Cre

Creatine

myo-I

Myo-inositol

Glu

Glutamate

Gln

Glutamine

GABA

γ-aminobutyric acid

DANTE

Delays alternating with nutations for tailored excitation

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Hui Mao
    • 1
  • Donna Toufexis
    • 2
  • Xiaoxia Wang
    • 1
  • Agnès Lacreuse
    • 2
    • 3
  • Shaoxiong Wu
    • 4
  1. 1.Department of Radiology and Frederick Philips MR Research CenterEmory University School of MedicineAtlantaUSA
  2. 2.Yerkes National Primate Research CenterEmory UniversityAtlantaUSA
  3. 3.Department of PsychologyUniversity of Massachusetts-AmherstAmherstUSA
  4. 4.Department of Chemistry and Center of NMR ResearchEmory UniversityAtlantaUSA