Review

European Food Research and Technology

, Volume 214, Issue 1, pp 3-26

First online:

Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products

  • Elke AnklamAffiliated withEuropean Commission, Joint Research Centre, Institute for Health and Consumer Protection, Food Products and Consumer Goods Unit, I-21020 Ispra, Italy
  • , Ferruccio GadaniAffiliated withPhilip Morris Europe, Research and Development, CH-2003 Neuchâtel, Switzerland
  • , Petra HeinzeAffiliated withEuropean Commission, Joint Research Centre, Institute for Health and Consumer Protection, Food Products and Consumer Goods Unit, I-21020 Ispra, Italy
  • , Hans PijnenburgAffiliated withPhilip Morris Europe, Research and Development, CH-2003 Neuchâtel, Switzerland
  • , Guy Van Den EedeAffiliated withEuropean Commission, Joint Research Centre, Institute for Health and Consumer Protection, Food Products and Consumer Goods Unit, I-21020 Ispra, Italy

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Abstract.

Numerous analytical methods, both qualitative and quantitative, have been developed to determine reliably the presence and/or the amount of genetically modified organisms (GMOs) in agricultural commodities, in raw agricultural materials and in processed and refined ingredients. In addition to the "classical" methods for DNA and protein analysis, e.g. polymerase chain reaction and enzyme linked immunosorbent analysis, certain types of GMO-containing matrices can be profiled by complementary chemical analysis methods such as chromatography and near infrared spectroscopy. This review summarises the status of the most widely used GMO analysis technologies, identifies new areas of analytical investigation and discusses current needs and future challenges.

Genetically modified organisms Polymerase chain reaction Enzyme-linked immunosorbent analysis Chromatography Near infrared spectroscopy