European Food Research and Technology

, Volume 236, Issue 2, pp 351–364

Biotyping Saccharomyces cerevisiae strains using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)

  • Anushka Moothoo-Padayachie
  • Himakar Reddy Kandappa
  • Suresh Babu Naidu Krishna
  • Thomas Maier
  • Patrick Govender
Original Paper

DOI: 10.1007/s00217-012-1898-1

Cite this article as:
Moothoo-Padayachie, A., Kandappa, H.R., Krishna, S.B.N. et al. Eur Food Res Technol (2013) 236: 351. doi:10.1007/s00217-012-1898-1

Abstract

Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS), a powerful biotyping tool for the identification of bacteria and clinical yeast isolates, was investigated as a method to rapidly identify industrial S. cerevisiae strains. In this study, an optimized sample preparation protocol was devised for the biotyping of S. cerevisiae strains. The results demonstrate that ethanol/formic acid protein extraction, of a cell density of 1 × 108 cells co-crystallized with α-cyano-4-hydroxycinnmaic acid, is required to yield mass spectral profiles that are diagnostic of each strain type. Forty-four S. cerevisiae strains commonly employed in South African fermentation-based industries were biotyped in an attempt to create a yeast reference database within a local context. The data revealed that MALDI-TOF MS can be used for the rapid and accurate identification of laboratory and industrial S. cerevisiae strains.

Keywords

BiotypingCommercial yeastMALDIS. cerevisiae database

Supplementary material

217_2012_1898_MOESM1_ESM.docx (213 kb)
Supplementary material 1 (DOCX 212 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Anushka Moothoo-Padayachie
    • 1
  • Himakar Reddy Kandappa
    • 1
  • Suresh Babu Naidu Krishna
    • 1
  • Thomas Maier
    • 2
  • Patrick Govender
    • 1
  1. 1.Faculty of Science and Agriculture, Department of BiochemistryUniversity of KwaZulu-NatalDurbanSouth Africa
  2. 2.Bruker Daltonik GmbHBremenGermany