Biotyping Saccharomyces cerevisiae strains using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
- First Online:
- Cite this article as:
- Moothoo-Padayachie, A., Kandappa, H.R., Krishna, S.B.N. et al. Eur Food Res Technol (2013) 236: 351. doi:10.1007/s00217-012-1898-1
- 458 Views
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS), a powerful biotyping tool for the identification of bacteria and clinical yeast isolates, was investigated as a method to rapidly identify industrial S. cerevisiae strains. In this study, an optimized sample preparation protocol was devised for the biotyping of S. cerevisiae strains. The results demonstrate that ethanol/formic acid protein extraction, of a cell density of 1 × 108 cells co-crystallized with α-cyano-4-hydroxycinnmaic acid, is required to yield mass spectral profiles that are diagnostic of each strain type. Forty-four S. cerevisiae strains commonly employed in South African fermentation-based industries were biotyped in an attempt to create a yeast reference database within a local context. The data revealed that MALDI-TOF MS can be used for the rapid and accurate identification of laboratory and industrial S. cerevisiae strains.