Original Paper

European Food Research and Technology

, Volume 236, Issue 1, pp 129-134

First online:

Development and application of highly specific PCR for detection of chicken (Gallus gallus) meat adulteration

  • Nagappa S. KarabasanavarAffiliated withDepartment of Veterinary Public Health and Epidemiology, Veterinary College Email author 
  • , S. P. SinghAffiliated withDepartment of Veterinary Public Health, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology
  • , Deepak KumarAffiliated withDepartment of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry
  • , Sunil N. ShebannavarAffiliated withGennova Biopharmaceutical

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In order to prevent fraud in the sale and strengthen quality assurance, authentic identification of chicken meat is essential. In the present investigation, a chicken (Gallus gallus)-specific polymerase chain reaction (PCR) was developed for the unambiguous identification of chicken meat. The PCR assay employs pair of primers designed against chicken nuclear 5-aminolevulinate (ALA) synthase gene. Highly chicken-specific diagnostic amplicon of 288 bp was established upon PCR and was evident in all the nine breeds/strains of chicken species. Sensitivity of PCR in detecting chicken meat adulteration was established to be at 0.1 % in the foreign meat matrix, while limit of detection (LOD) of chicken DNA was 10 pg. Suitability of the developed chicken-specific PCR was validated and confirmed in raw, cooked/heat treated (60, 80, 100, and 121 °C), and micro-oven cooked meat samples. Possibility of cross-amplification of adulterating DNA was excluded by cross-checking the developed PCR assay with several animal and avian species. The PCR assay developed in this study is highly promising for applications involving circumstances that require authentic identification of chicken meat.


Meat Chicken Adulteration DNA PCR