European Food Research and Technology

, Volume 236, Issue 1, pp 129–134

Development and application of highly specific PCR for detection of chicken (Gallus gallus) meat adulteration

  • Nagappa S. Karabasanavar
  • S. P. Singh
  • Deepak Kumar
  • Sunil N. Shebannavar
Original Paper

DOI: 10.1007/s00217-012-1868-7

Cite this article as:
Karabasanavar, N.S., Singh, S.P., Kumar, D. et al. Eur Food Res Technol (2013) 236: 129. doi:10.1007/s00217-012-1868-7


In order to prevent fraud in the sale and strengthen quality assurance, authentic identification of chicken meat is essential. In the present investigation, a chicken (Gallus gallus)-specific polymerase chain reaction (PCR) was developed for the unambiguous identification of chicken meat. The PCR assay employs pair of primers designed against chicken nuclear 5-aminolevulinate (ALA) synthase gene. Highly chicken-specific diagnostic amplicon of 288 bp was established upon PCR and was evident in all the nine breeds/strains of chicken species. Sensitivity of PCR in detecting chicken meat adulteration was established to be at 0.1 % in the foreign meat matrix, while limit of detection (LOD) of chicken DNA was 10 pg. Suitability of the developed chicken-specific PCR was validated and confirmed in raw, cooked/heat treated (60, 80, 100, and 121 °C), and micro-oven cooked meat samples. Possibility of cross-amplification of adulterating DNA was excluded by cross-checking the developed PCR assay with several animal and avian species. The PCR assay developed in this study is highly promising for applications involving circumstances that require authentic identification of chicken meat.



Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Nagappa S. Karabasanavar
    • 1
  • S. P. Singh
    • 2
  • Deepak Kumar
    • 3
  • Sunil N. Shebannavar
    • 4
  1. 1.Department of Veterinary Public Health and EpidemiologyVeterinary CollegeShimogaIndia
  2. 2.Department of Veterinary Public Health, College of Veterinary and Animal SciencesG. B. Pant University of Agriculture and TechnologyPantnagarIndia
  3. 3.Department of Veterinary Public Health and EpidemiologyCollege of Veterinary Science and Animal HusbandryAgartalaIndia
  4. 4.Gennova BiopharmaceuticalPuneIndia