European Food Research and Technology

, Volume 232, Issue 5, pp 769–776

Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of Staphylococcus aureus in food

  • Hong Yang
  • Xiaoyan Ma
  • Xianzhou Zhang
  • Yu Wang
  • Wei Zhang
Original Paper

DOI: 10.1007/s00217-011-1442-8

Cite this article as:
Yang, H., Ma, X., Zhang, X. et al. Eur Food Res Technol (2011) 232: 769. doi:10.1007/s00217-011-1442-8

Abstract

A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Staphylococcus aureus in food was developed and evaluated. Heat-stable nuclease (nuc) gene was the target sequence amplified by LAMP. LAMP includes primer design, amplification at 64 °C, macroscopic observation, and estimation of the results. The LAMP detection of S. aureus was sensitive up to 1.25 CFU per reaction tube, with a detection limit of 10.3 CFU per reaction tube in the artificial contamination test, and the experiment took less than 1 h. Sensitivity of the method in Staphylococcus aureus detection was 100%, with 97.93% specificity and a 98.5% match ratio when compared using Chinese National Standard. The LAMP method is superior to the Chinese National Standard method in terms of sensitivity, cost, testing time, and convenience.

Keywords

Loop-mediated isothermal amplificationLAMPDetectionStaphylococcus aureusFood

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Hong Yang
    • 1
  • Xiaoyan Ma
    • 1
  • Xianzhou Zhang
    • 1
  • Yu Wang
    • 1
  • Wei Zhang
    • 1
  1. 1.College of Food Science and TechnologyAgricultural University of HeBeiBaodingPeople’s Republic of China